Recombinant Rabbit Anti-SPTAN1 Antibody (clone R08-7C6) (CAT#: VS3-FY1385)
This product is a recombinant rabbit antibody that recognizes SPTAN1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R08-7C6 is specific for human, mouse, rat SPTAN1. The antigen is synthetic peptides of human neas.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to microtubules & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7927/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to microtubules & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7927/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_B_7_5.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_B_7_5.jpg
Figure 3 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_7_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_7_3.jpg
Figure 4 Pancreas
(Exocrine glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_1_3.jpg
(Exocrine glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_1_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_8_5.jpg
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_8_5.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197694-SPTAN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197694-SPTAN1
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197694-SPTAN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197694-SPTAN1
Specifications
- Immunogen
- Synthetic peptides of human NEAS.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat SPTAN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-7C6
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 285 kDa; Observed MW: 285 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Spectrin Alpha, Non-Erythrocytic 1; Spectrin, Non-Erythroid Alpha Subunit; Fodrin Alpha Chain; Alpha-II Spectrin; Alpha-Fodrin; SPTA2
- Gene ID
- 6709
- UniProt ID
- Q13813
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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