Mouse Anti-TH Recombinant Antibody (clone 2506W0057)
CAT#: VS-0625-FY162
This antibody targets Tyrosine Hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis. TH catalyzes the conversion of tyrosine to L-dihydroxyphenylalanine (L-Dopa) and is crucial for the production of dopamine, noradrenaline, and adrenaline. The antibody, produced in mice and reactive with human samples, is applicable in ELISA, aiding researchers in studying TH's function in neurotransmitter biosynthesis and related neurological disorders.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14010/1905_B8_3_selected.jpg
Normal Tissue
Figure 2 High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/159875_B_6_5_val_selected.jpg
Normal Tissue
Figure 3 Low expression in pancreas
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/160099_A_3_3_val_selected.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (PC-3, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000180176-TH
❮
❯
Specifications
- Immunogen
- Human TH
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The antibody specifically recognizes the peptide of the Human TH.
- Species Reactivity
- Human
- Clone
- 2506W0057
- Applications
- ELISA
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- The buffer commonly used is typically composed of 1 M Tris-HCl and 100 mM glycine. For more detailed information, refer to the Certificate of Analysis (COA).
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1:1000-1:3000
Other applications are under development. Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Tyrosine Hydroxylase; Tyrosine 3-Monooxygenase; Tyrosine 3-Hydroxylase; EC 1.14.16.2; TYH; Dystonia 14; EC 1.14.16; DYT14; DYT5b
- Gene ID
- 7054
- UniProt ID
- P07101
- Long Name
- Tyrosine 3-monooxygenase
- Sequence Similarities
- Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoplasmic vesicle, Nucleus, Synapse
- Post Translation Modifications
- Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities.
Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013).
Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903).
Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway.
Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871).
Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).
Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity.
- Protein Refseq
- XP_011518637.1; XP_054225745.1; NP_000351.2; NP_954986.2; NP_954987.2
- Function
- Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0625-FY162. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Amphetamine Addiction
Alcoholism
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "TH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2042z | Mouse Anti-TH Recombinant Antibody (clone 26H11) | WB, ICC, IF | Mouse IgG2a |
| MRO-2249-CN | Rabbit Anti-TH Polyclonal Antibody (MRO-2249-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ456 | Rabbit Anti-TH Recombinant Antibody (VS3-CJ456) | WB, ICC, IHC, FC | Rabbit IgG |
| VS3-FY1925 | Rabbit Anti-TH Recombinant Antibody (clone R01-3E4) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
| VS7-0425-WR979 | Mouse Anti-TH Recombinant Antibody (clone 1B8D2) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0734MZ | Chicken Anti-TH Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3558 | Rabbit Anti-TH Recombinant Antibody (clone DS3558AB) | WB, FC | Rabbit IgG |
| MOR-4763 | Rabbit Anti-TH Recombinant Antibody (clone TH277DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0099-FY | Rabbit Anti-TH Recombinant Antibody (clone AFY0070) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1269 | PE-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1270 | APC-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1271 | FITC-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
| MHC-CN1272 | H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC381 | AbPlus™ Anti-TH Magnetic Beads (VS-0724-YC381) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2280 | Anti-TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7225 | Anti-Mouse TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7224 | Anti-Human TH Immunohistochemistry Kit | IHC | |
| VS-0525-XY7226 | Anti-Rat TH Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
-2.png)
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, WB, BLI, SPR
Application: ELISA, IHC, FC, IP, IF, FuncS
-2.png)
Application: ELISA
Application: Inhib, FC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
