Mouse Anti-TH Recombinant Antibody (clone 2506W0057) (CAT#: VS-0625-FY162)

• Immunogen
• Human TH

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• The antibody specifically recognizes the peptide of the Human TH.

• Species Reactivity
• Human

• Clone
• 2506W0057

• Applications
• ELISA

• Purification
• Purified with Protein A or G affinity chromatography

• Purity
• >90% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• The buffer commonly used is typically composed of 1 M Tris-HCl and 100 mM glycine. For more detailed information, refer to the Certificate of Analysis (COA).

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• ELISA: 1:1000-1:3000
  Other applications are under development. Optimal dilutions for assays should be determined by the user.

• Alternative Names
• Tyrosine Hydroxylase; Tyrosine 3-Monooxygenase; Tyrosine 3-Hydroxylase; EC 1.14.16.2; TYH; Dystonia 14; EC 1.14.16; DYT14; DYT5b

• Gene ID
• 7054

• UniProt ID
• P07101

• Long Name
• Tyrosine 3-monooxygenase

• Sequence Similarities
• Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

• Cellular Localization
• Cell projection, Cytoplasm, Cytoplasmic vesicle, Nucleus, Synapse

• Post Translation Modifications
• Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities.
  Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013).
  Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903).
  Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway.
  Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871).
  Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).
  Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity.

• Protein Refseq
• XP_011518637.1; XP_054225745.1; NP_000351.2; NP_954986.2; NP_954987.2

• Function
• Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

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