Creative Biolabs provides a wide range of services related to antibody-liposome production, providing customized solutions based on specific needs. We offer a range of breast-targeting antibody conjugates, such as monoclonal and polyclonal antibodies. Our customer service team is ready to assist with any inquiries related to product application, storage, and experimental design.

Introduction

Targeted breast cancer liposomes are a type of nanocarrier primarily designed for drug delivery, particularly in the treatment of breast cancer. Typically, drugs can be encapsulated within these liposomes, providing protection for the drug's stability within the body and allowing for sustained release. The targeting capabilities can be enhanced by modifying the surface of the liposomes with specific ligands that selectively bind to receptors on the surface of breast cells, enabling precise targeting.

Creative Biolabs offers antibody screening and optimization services, utilizing high-throughput screening technology to identify antibodies with specific targeting capabilities. Nowadays, we are capable of designing and preparing liposomes to ensure the efficiency and stability of drug encapsulation. Furthermore, we can conduct pharmacodynamic studies using animal models to evaluate the efficacy and safety of antibody-liposome conjugates. At the same time, our toxicological analyses can assist clients in establishing appropriate dosage ranges and assessing potential side effects and toxic reactions. Also, we provide pharmacokinetic and biodistribution studies, using specialized equipment and techniques to evaluate the distribution and metabolism of antibody-liposomes in the body.

Fig.1 A Lipid-peptide Conjugated HER2-Breast Targeting Antibody-coupled Liposome.^1,3

1. Target Selection and Antibody Screening: Our team will work closely with clients to identify the best targets and corresponding antibodies to ensure the precision of the treatment.

2. Liposomal Preparation: Based on the selected antibodies, we will prepare corresponding targeted liposomes, ensuring they possess ideal physicochemical properties for effective cellular uptake.

3. Conjugation Reaction Optimization: In the laboratory, we will conduct conjugation reactions between antibodies and liposomes, continuously optimizing the reaction conditions to achieve the best conjugation efficiency and stability.

4. Functional Verification: Through cellular experiments and animal models, we will validate the targeting capability and therapeutic effect of the conjugated products, ensuring their safety and efficacy.

5. Preclinical Research Support: We offer comprehensive preclinical research support, including pharmacokinetics, pharmacodynamics, and safety assessments, to assist clients in preparing for clinical trials.

Services

Creative Biolabs combines antibody technology with liposome delivery systems, effectively leveraging the advantages of both. Our antibodies can specifically recognize antigens on the surface of breast cells, enabling precise targeting. Meanwhile, liposome products can efficiently carry drugs, protecting their stability in the body and enhancing their biodistribution and cellular uptake. By conjugating antibodies with liposomes, we can deliver therapeutic agents directly to tumor cells, improving treatment efficacy while minimizing damage to normal tissues.

In our labs, antibody-coupled liposomes designed for targeted breast therapy serve as an effective drug delivery system, enhancing the therapeutic effects of targeted drugs while minimizing damage to healthy tissues. Typically, we select phospholipids (such as DPPC and DOPC) and cholesterol, dissolved in a chloroform-methanol mixture, to create lipid membranes. The phospholipids and cholesterol are mixed in proportion and subjected to rotary evaporation to remove the organic solvent, resulting in the formation of lipid films. We then add physiological saline or PBS to the prepared lipid films and use ultrasonic treatment to generate lipid nanoparticles. To conjugate antibodies with ligands modified on the lipid nanoparticle surface, we commonly employ chemical methods such as the EDC/NHS coupling technique.

Fig.2 In Vivo HER2-targeted PET Imaging Analysis.^2,3

• Liposomes Preparation and Characterization Services:

Design and optimize liposome formulations targeted for breast tissue.

Employ various techniques (such as film hydration and ultrasound methods) to fabricate the liposomes.

Characterize their physicochemical properties, including particle size, turbidity, and Zeta potential.

• Drug Loading and Release Studies:

Evaluate the drug loading efficiency and capacity within the liposomes.

Investigate the release kinetics of the drug under different conditions.

• Cytotoxicity and Biocompatibility Testing:

Assess the cytotoxicity of the targeted liposomes using in vitro cell models.

Conduct biocompatibility tests to determine the impact of the liposomes on normal cells.

• Cell Uptake and Targeting Studies:

Evaluate the uptake of targeted liposomes in specific breast cancer cell lines.

Analyze targeting efficiency using techniques like flow cytometry or fluorescence microscopy.

• Animal Model Experiments Assays:

Test the pharmacodynamics of targeted liposomes in mouse models of breast cancer.

Assess the in vivo distribution, metabolism, and excretion characteristics of the drug.

• Toxicological Analysis Systems:

Conduct a systematic toxicological evaluation, including acute and chronic toxicity tests.

Evaluate the effects on major organs and potential side effects.

• Pharmacokinetic Studies:

Investigate the pharmacokinetic properties of targeted liposomes in vivo, focusing on absorption, distribution, metabolism, and excretion (ADME).

Creative Biolabs employs advanced antibody liposome preparation techniques to assist clients in selecting the appropriate liposomes and production strategies. The exact duration varies depending on the type of product and specific requirements, usually ranging from a few weeks to several months. Throughout this process, we ensure that customers receive detailed instructions and technical support to facilitate smooth usage of the product. If you have any specific needs or questions, please feel free to contact us we are here to assist you.

For research use only. Not intended for any clinical use.