pH-Sensitive Antibody: Advance in Antibody Development

pH-sensitive antibodies are novel therapeutics that specifically target antigens in a pH-dependent manner. The mechanism reveals that pH-sensitive antibodies bind strongly in the circulation and dissociate in acidic circumstances, increasing efficacy while decreasing off-target effects, and extending the half-life of monoclonal antibodies in vivo. As a result, further research on pH-sensitive antibodies is necessary for efficient antibody therapeutic development.

Fig.1 pH-sensitive antibodies' dependent action.¹

pH-Sensitive Antibody Production and QC Service at Creative Biolabs

Creative Biolabs offers a customized pH-sensitive antibody production and QC service for specific antibody generation. Our advanced platforms enable the precise engineering of antibodies with tailored pH-dependent binding properties. By incorporating pH-sensitive amino acid residues or motifs, we can modulate antibody affinity to target specific pH environments. Our solutions extend to the optimization of expression systems, including mammalian and insect cells, to ensure optimal protein folding and post-translational modifications.

The purification of pH-sensitive antibodies demands meticulous attention to preserve their unique properties. We leverage advanced purification techniques, including pH-dependent hydrophobicity chromatography and ion exchange chromatography, to capitalize on the differential charge and hydrophobicity of antibodies at various pH levels. Our proprietary pH-responsive affinity ligands bind to antibodies at neutral pH and release them in acidic conditions, enabling efficient and gentle purification. Additionally, we offer traditional methods like protein A/G affinity chromatography and size-exclusion chromatography for comprehensive purification.

Throughout the entire process, we adhere to stringent guidelines and maintain detailed records of all production and quality control activities. Rigorous characterization, including evaluation of general properties, pH sensitivity, and functionality, ensures the delivery of high-quality pH-sensitive antibodies.

Fig.2 Our service process.

Eukaryotic Expression Systems for High-quality pH-Sensitive Antibody Expression

The choice of expression system significantly impacts the yield, quality, and post-translational modifications of the expressed antibody. We have validated several expression systems that are suitable for pH-sensitive antibody production, including:

In addition to providing mammalian cell line expression systems, we also provide other related systems, such as Sf9 cell, etc.

Quality Considerations

• pH-Sensitivity Characterization: Binding Affinity; pH-Dependent Conformational Changes; Stability at Different pH
• Post-Translational Modifications
• Purity and Homogeneity
• Protein Functionality
• Stability and Storage

Our Advantages

• Precise pH control
• Proprietary purification technologies supplied
• Comprehensive quality considerations
• Robust antibody production platform
• Competent experts team
• One-stop customized services

FAQ

Q1: What are the costs associated with pH-sensitive antibody production services?

A1: The cost of pH-sensitive antibody production can vary based on factors like the complexity of the antibody, the quantity required, and the level of customization. Please contact us for a detailed quote.

As a leading provider of antibody solutions, Creative Biolabs is committed to advancing the development and production of pH-sensitive antibodies. We continuously refine our processes to deliver high-quality antibodies efficiently and reliably. If you are looking for a trustworthy companion for your pH-sensitive antibody projects, please contact us immediately.

For research use only. Not intended for any clinical use.