Recombinant Rabbit Anti-ACKR3 Antibody (clone R08-6H8)
CAT#: VS3-FY15
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ACKR3 protein. It can detect a band of approximately 41 kDa (observedd molecular weight: 41 kDa).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32003/1048_F7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblasts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_2_7_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32003/1047_F7_1_blue_red_green.jpg
Normal Tissue
Figure 4 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_1_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HeLa, MCF7, RT4, SiHa, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144476-ACKR3
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WB
Figure 1. Recombinant Rabbit Anti-ACKR3 Antibody (clone R08-6H8) in WB.
Western blot analysis of GPCR RDC1/CXCR7 in K562, C6, 3T3, Hela lysates using RDC1 Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human GPCR RDC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACKR3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-6H8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 41 kDa; Observed MW: 41 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Atypical Chemokine Receptor 3; Chemokine Orphan Receptor 1; G-Protein Coupled Receptor RDC1 Homolog; Chemokine (C-X-C Motif) Receptor 7; C-X-C Chemokine Receptor Type 7; G-Protein Coupled Receptor 159; CMKOR1; CXC-R7
- Gene ID
- 57007
- UniProt ID
- P25106
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.
- Cellular Localization
- Cell membrane, Endosome, Membrane
- Post Translation Modifications
- The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated.
Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.
- Protein Refseq
- NP_064707.1
- Function
- Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed:16107333, PubMed:19255243, PubMed:19380869, PubMed:20161793, PubMed:22300987).
Chemokine leads to ligand internalization and activation of MAPK signaling pathway (PubMed:16940167, PubMed:18653785, PubMed:20018651).
Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed:16940167, PubMed:18653785).
In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed:16940167, PubMed:20388803).
Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed:17804806, PubMed:18653785, PubMed:19641136, PubMed:20887389).
Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed:18653785).
Required for heart valve development (PubMed:17804806).
Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed:31211835).
(Microbial infection) Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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