Recombinant Rabbit Anti-ACKR3 Antibody (clone R08-6H8) (CAT#: VS3-FY15)

• Immunogen
• A synthetic peptide of human GPCR RDC1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat ACKR3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R08-6H8

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• Calculated MW: 41 kDa; Observed MW: 41 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Atypical Chemokine Receptor 3; Chemokine Orphan Receptor 1; G-Protein Coupled Receptor RDC1 Homolog; Chemokine (C-X-C Motif) Receptor 7; C-X-C Chemokine Receptor Type 7; G-Protein Coupled Receptor 159; CMKOR1; CXC-R7

• Gene ID
• 57007

• UniProt ID
• P25106

• Sequence Similarities
• Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.

• Cellular Localization
• Cell membrane, Endosome, Membrane

• Post Translation Modifications
• The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated.
  Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.

• Protein Refseq
• NP_064707.1

• Function
• Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed:16107333, PubMed:19255243, PubMed:19380869, PubMed:20161793, PubMed:22300987).
  Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed:16940167, PubMed:18653785, PubMed:20018651).
  Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed:16940167, PubMed:18653785).
  In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed:16940167, PubMed:20388803).
  Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed:17804806, PubMed:18653785, PubMed:19641136, PubMed:20887389).
  Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed:18653785).
  Required for heart valve development (PubMed:17804806).
  Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed:31211835).
  (Microbial infection) Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody