Recombinant Rabbit Anti-CASP1 Antibody (clone R05-7H1) (CAT#: VS3-FY190)

• Immunogen
• Recombinant protein of human Caspase-1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CASP1

• Species Reactivity
• Human

• Clone
• R05-7H1

• Applications
• Western Blot, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 45 kDa; Observed MW: 45,20 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• Caspase 1; Caspase 1, Apoptosis-Related Cysteine Peptidase; Interleukin 1, Beta, Convertase; IL-1 Beta-Converting Enzyme; EC 3.4.22.36; Caspase-1; IL1BC; P45; ICE; Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase); Caspase 1, Apoptosis-Related Cysteine Protease (Interleukin 1, Beta, Convertase)

• Gene ID
• 834

• UniProt ID
• P29466

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cell membrane, Cytoplasm, Membrane

• Post Translation Modifications
• The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8.
  Cleavage in the interdomain linker region is required to induce pyroptosis.

• Protein Refseq
• NP_001214.1; NP_001244047.1; NP_001244048.1

• Function
• Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255).
  Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255).
  Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465).
  Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003).
  In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275).
  Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:22464733).
  Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
  In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).
  Isoform Delta: Apoptosis inactive.
  Isoform Epsilon: Apoptosis inactive.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody