APC-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer

Name: APC-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer
SKU: MHC-LC067
Rating: 5 (1 reviews)

CAT#: MHC-LC067

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of CD20-derived peptide of SLFLGILSV sequence covering 188-196 and A*02:01 molecule. The pMHC tetramer recognizes human CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Allele
A*02:01

Class
Class I

MHC Species
Human

Antigen
CD20

Antigen Species
Human

Peptide
SLFLGILSV

Range
188-196

Conjugate
APC

Application
FCM

Target

Antigen Introduction
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.

Alternative Names
MS4A1; membrane spanning 4-domains A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Gene ID
911829

UniProt ID
Q05320

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Datasheet

Datasheet - MHC-LC067.pdf

MSDS

MSDS - MHC-LC067.pdf

COA

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