SmartAb™ Recombinant Anti-MS4A1 pH-dependent Antibody (VS-0825-YC17)

Name: SmartAb™ Recombinant Anti-MS4A1 pH-dependent Antibody (VS-0825-YC17)
SKU: VS-0825-YC17
Rating: 5 (1 reviews)

CAT#: VS-0825-YC17

The anti-MS4A1 pH-dependent antibody (VS-0825-YC17) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human MS4A1. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for MS4A1 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from MS4A1 at pH 6.0. This pH-sensitive antibody retains its binding affinity for MS4A1 on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human MS4A1, also known as CD20, is a transmembrane protein expressed on the surface of B-cells. It plays a crucial role in B-cell development and activation, making it a prominent target for antibody-based therapies in B-cell lymphomas and autoimmune diseases.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

❮

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Specifications

Host Species
Human

Derivation
Humanized (from mouse)

Type
Human IgG1 kappa

Specificity
Human MS4A1

Species Reactivity
Human

Applications
FC, Inhib

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE and SEC-HPLC

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.

Target

Alternative Names
MS4A1; membrane-spanning 4-domains, subfamily A, member 1; CD20; B-lymphocyte antigen CD20; B1; Bp35; CD20 antigen; CD20 receptor

Gene ID
931

UniProt ID
P11836

Long Name
Membrane Spanning 4-Domains A1

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0825-YC17.pdf

MSDS

MSDS - VS-0825-YC17.pdf

COA

Certificate of Analysis Lookup

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