Human Anti-MS4A1 Recombinant Antibody (clone 2F2)

Name: Human Anti-MS4A1 Recombinant Antibody (clone 2F2)
SKU: PABL-422
Rating: 5 (1 reviews)

CAT#: PABL-422

Recombinant Human Antibody (2F2) is capable of binding to CD20, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CD20 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CD20 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody is a fully human anti-CD20 antibody that is currently in phase III clinical trial for several types of malignancies and autoimmune diseases and is one of the most promising antiCD20 drugs.

Tested Data Published Data Gene Expression

SDS-PAGE

Figure 1 Human Anti-MS4A1 Recombinant Antibody (PABL-422) in SDS-PAGE

Lane 1: Non-reduced
Lane 2: Reduced

SEC-HPLC

Figure 2 Human Anti-MS4A1 Recombinant Antibody (PABL-422) in HPLC

HPLC analysis of PABL-422 shows the purity is>95%.

Figure 3 Human Anti-MS4A1 Recombinant Antibody (PABL-422) in Flow Cytometry

Daudi cells were stained with Anti-CD20 Antibody (Cat# PABL-422, Blue line) or isotype control antibody (Yellow line), followed by FITC-conjugated Goat Anti-human IgG Secondary Antibody.

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Figure 1 Western blot analysis of purified anti-CD20 mAbs separated by SDS-PAGE.

Lane1, molecular weight protein markers; lane2, C2B8; lane3, 2F2; lane4, C2B8(ScFvHL)4-Fc; lane5, 2F2(ScFvHL)4-Fc.

Li, B., Shi, S., Qian, W., Zhao, L., Zhang, D., Hou, S., ... & Guo, Y. (2008). Development of novel tetravalent anti-CD20 antibodies with potent antitumor activity. Cancer research, 68(7), 2400-2408.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
Human membrane-spanning 4-domains, subfamily A, member 1

Host Species
Human

Derivation
Human

Type
Human IgG1,κ

Specificity
CD20

Species Reactivity
Human

Clone
2F2

Applications
WB, ELISA, FuncS

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;

Gene ID
931

UniProt ID
P11836

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(Creative Biolabs Cat# PABL-422, RRID: AB_3111657)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABL-422.pdf

MSDS

MSDS - PABL-422.pdf

COA

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