Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-028)

Name: Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-028)
SKU: AFC-TAB-028
Rating: 5 (1 reviews)

CAT#: AFC-TAB-028

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human CD20. It is a human monoclonal antibody (for the CD20 protein) which appears to inhibit early-stage B lymphocyte activation.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
Hmuan CD20 transfected NS/O cells.

Host Species
Human

Derivation
Human

Type
ADCC enhanced antibody

Antibody Isotype
IgG1, κ

Species Reactivity
Human

Applications
FC, IP, ELISA, Neut, FuncS, IF

Conjugate
Unconjugated

Trade name
Arzerra

Generic Name
Ofatumumab

Related Disease
Rheumatoid arthritis (RA)

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
MS4A1; membrane-spanning 4-domains, subfamily A, member 1; CD20; B-lymphocyte antigen CD20; B1; Bp35; MS4A2; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16

Gene ID
931

UniProt ID
P11836

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Biosimilar Overview

Please refer to Ofatumumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ofatumumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-028.pdf

MSDS

MSDS - AFC-TAB-028.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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Ofatumumab is an anti-CD20 antibody used in the treatment of chronic lymphocytic leukemia (CLL) for patients with particular treatment histories and specific responsiveness to anticancer therapies. The DrugMonitor™ Anti-Ofatumumab Antibody (VS-1224-YC836) is an anti-drug antibody (ADA) against Ofatumumab. This drug-based antibody is raised in mice immunized with the Ofatumumab. The anti-Ofatumumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Ofatumumab in samples.

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