Mouse Anti-CD28 Recombinant Antibody (clone 1C6)
CAT#: PABL-365
Recombinant Mouse Antibody (1C6) is capable of binding to Canine CD28. This antibody could inhibit MLRs at 20 nanomolar concentrations as effectively as CTLA4-Ig, suggesting that the binding site of the mAb inhibits CD28 interaction with CD80/CD86.
Published Data
Gene Expression
Figure 1 Histopathology of 4 dogs treated with anti-CD28 (1C6) mAb.
The top left panel shows H520 necropsy lung section at 100× magnification demonstrating edema (top arrow), dense lymphocytic infiltrate (middle arrow), and hemorrhage (bottom arrow). The middle left panel shows H520 necropsy lung section at 250× magnification after antimouse peroxidase polymer staining of deparaffinized lung section (arrow points to pulmonary macrophages coated with anti-CD28 mAb). The bottom left panel shows 250× magnification of antirabbit peroxidase polymer control. The top middle panel shows H517 necropsy small intestine section at 100× magnification demonstrating significant hemorrhage on the tips of the villi. The center middle row shows normal small bowel at100× magnification for comparison. The bottom center panel shows H208 necropsy small intestine section at 100× magnification; the arrow points to an enlarged and densely packed Peyer patch. The top right and middle right panel shows H514 necropsy lymph node section at 100× (top) and 250× (middle) magnifications; the arrows point to apoptotic bodies. Slides are stained by hematoxylin and eosin unless otherwise noted.
Rosinski, S. L., Storb, R., Strong, R. K., Sale, G. E., Stone, D. M., Gewe, M. M., ... & Graves, S. S. (2015). Anti-CD28 antibody-initiated cytokine storm in canines. Transplantation direct, 1(2).
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Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Immunogen
- Canine Cluster of Differentiation 28
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- canine CD28
- Species Reactivity
- canine
- Clone
- 1C6
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cluster of Differentiation 28; CD28
- Gene ID
- 403646
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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