Recombinant Mouse Antibody (Ab-52) is capable of binding to Ft LPS , expressed in Chinese Hamster Ovary cells (CHO). The epitope recognized by Ab52 is contained within two internal repeat units of the O-polysaccharide [O-antigen (OAg)] of Ft LPS.
Figure 1 Ab52 bind to Ft LPS by Western blots.
Ft LPS (200 μg, usually 10-20 μg) was electrophoresed in a pre-parative 4-15% polyacrylamide gel under denaturing conditions. After transfer to nitrocellulose, strips were probed with the Ab 52 at the indicated concentrations and developed with AP-anti-mouse IgG (H +L) conjugate for 15 min.
Lu, Z., Rynkiewicz, M. J., Yang, C. Y., Madico, G., Perkins, H. M., Roche, M. I., ... & Sharon, J. (2014). Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclonal antibodies in immunodiagnosis and immunotherapy, 33(4), 235-245.
Figure 2 The three new MAbs bind specifically to virulent Ft in ELISA compared with Ab52.
ELISA plates were coated with Ft LPS and were probed with serial dilutions of the indicated MAbs. GTX40330, a commercial MAb specific for E. coli LPS, was used as specificity control.
Lu, Z., Rynkiewicz, M. J., Yang, C. Y., Madico, G., Perkins, H. M., Roche, M. I., ... & Sharon, J. (2014). Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclonal antibodies in immunodiagnosis and immunotherapy, 33(4), 235-245.
Figure 3 Binding of N203 to Ft SchuS4 vortexate is inhibited by OAgC and by internal binding but not terminal binding anti-OAg MAbs.
Serially diluted Ft OAgC was pre-mixed with 5 mg/mL N203, Ab52, or Ab53 (anti-Ft GroEL, IgG2a isotype control); then the mixtures were added to a SchuS4 vortexate-coated, washed, and blocked ELISA plate, followed by HRP-a-m-IgG2a to detect bound MAb.
Lu, Z., Rynkiewicz, M. J., Yang, C. Y., Madico, G., Perkins, H. M., Roche, M. I., ... & Sharon, J. (2014). Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclonal antibodies in immunodiagnosis and immunotherapy, 33(4), 235-245.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-630 | Recombinant Anti-LPS Antibody | ELISA, WB, FC, FuncS | IgG |
PABW-013 | Mouse Anti-LPS Recombinant Antibody (clone S45-18) | WB, ELISA | Mouse IgG |
PABZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody (SYA/J6) | WB, FuncS | IgG |
PABW-168 | Mouse Anti-LPS Recombinant Antibody (clone F22-4) | ELISA | Mouse IgG |
FAMAB-0104-CN | Recombinant Human Anti-LPS Antibody (S20) | ELISA, FC, BL | Human IgG2 |
CAT | Product Name | Application | Type |
---|---|---|---|
NABL-060 | Recombinant Anti-V. cholerae O1 LPS VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-630-S(P) | Recombinant Human Anti-LPS Antibody scFv Fragment | IF, Neut, FuncS | scFv |
PSBL-265 | Recombinant Mouse Anti-LPS Antibody scFv Fragment (S25-2) | WB, IHC, FuncS | scFv |
PSBZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody scFv Fragment (SYA/J6) | WB, FuncS | scFv |
PSBW-168 | Mouse Anti-LPS Recombinant Antibody (clone F22-4); scFv Fragment | ELISA | Mouse scFv |
FAMAB-0087WJ-S(P) | Human Anti-LPS Recombinant Antibody (clone HR78); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-078 | Recombinant Mouse Anti-E. coli LPS Antibody Fab Fragment (WN1 222-5) | WB, ELISA, FuncS | Fab |
PFBZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody Fab Fragment (SYA/J6) | WB, FuncS | IgG |
PFBL-474 | Recombinant Mouse Anti-Ft LPS Antibody Fab Fragment (Ab-52) | WB, ELISA, FuncS | Fab |
FAMAB-0244-CN-F(E) | Recombinant Chimeric (Mouse/Human) Anti-LPS Antibody Fab Fragment (CK3) | WB, ELISA | Chimeric (mouse/human) Fab |
FAMAB-0245-CN-F(E) | Recombinant Mouse Anti-LPS Antibody Fab Fragment (BML-5D11) | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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