Recombinant monoclonal antibody to HER2/neu. This antibody intended for the prophylaxis and treatment of ovarian carcinoma xenografts.
Figure 1 Flow cytometry analysis of MIL5 binding affinity.
Flow cytometry analysis showed dose-dependent binding of MIL5 to four HER2+ cell lines: SKOV3, N87, MDA-MB-175 and SKBR3.
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 2 MIL5 and 2C4 could inhibit each other in HER2 binding.
MIL5 can inhibit 2C4_FITC with the IC₅₀ of 2.7 μg/ml (SKOV3) and 3.69 μg/ml (SKBR3).
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 3 ADCC and receptor-induced internalization of MIL5.
Internalization of MIL5 in HER2-positive SKOV3 and SKBR3 cells. More antibody molecules entered SKOV3 cells (3 x 10⁶) than SKBR3 (1.2 x 10⁵), and MIL5 seemed to have weaker internalization function than 2C4 in SKBR3 cells.
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 4 ADCC and receptor-induced internalization of MIL5.
ADCC detection of MIL5 in SKOV3 and SKBR3 cells on a dose-dependent manner. MIL5 induced a better ADCC effect than 2C4. A human anti-CD20 monoclonal antibody (TGLA) was set as isotype control (negative).
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 5 MIL5 blocks the downstream signal cascades of HER family to inhibit the growth/amplification of cancer.
Both ERK and AKT pathways were inhibited by MIL5 in HER1/HER2+ SKOV3 and HER2/HER3+ N87 cells.
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 6 MIL5 induces apoptosis and arrested cancer cells in G2 phase identified by multiparametric assays.
A total actin increased and aggregated on a dosedependent manner.
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
Figure 7 Flow cytometry analysis of MIL5 binding affinity.
MIL5 and 2C4 could bind ECD and ECDm311 mutants but not ECDm235.
Qiao, C., Lang, X., Luo, L., Geng, S., Lv, M., Geng, J., ... & Li, Y. (2017). Multi-parametric analysis reveals enhanced G2-phase arrest of an optimized anti-HER2 antibody to inhibit breast cancer. Biotechnology letters, 39(9), 1309-1323.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-059 | Recombinant Anti-Human ERBB2 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
TAB-032CT | Anti-Human HER2/neu Therapeutic Single Domain Antibody | WB | Single domain antibody |
TAB-046CT | Anti-Human HER2/neu Therapeutic Single Domain Antibody (Gr3) | ELISA, Inhibition, FC | Single domain antibody |
TAB-047CT | Anti-Human HER2/neu Therapeutic Single Domain Antibody (Gr6) | WB | Single domain antibody |
HPAB-1026WJ | Recombinant Camelid Anti-ERBB2 Single Domain Antibody | ELISA | Camelid VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-463 | Human Anti-ERBB2 Recombinant Antibody (clone mAb37); Fab Fragment | WB | Human Fab |
HPAB-0608LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0608LY-F(E)) | ELISA, Inhib | Humanized Fab |
HPAB-0609LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0609LY-F(E)) | ELISA, Inhib | Humanized Fab |
HPAB-0610LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0610LY-F(E)) | ELISA, Inhib | Humanized Fab |
HPAB-M0510-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0510-YC-F(E)) | FC, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0191CL-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-0191CL-F(E)) | ELISA, Cyt, Internalization | Human Fab |
TAB-0192CL-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-0192CL-F(E)) | ELISA, Cyt, Internalization | Human Fab |
TAB-040CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (C6.5) | FC, IP, Block | Human antibody |
TAB-042CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (CRX01) | WB | Human antibody |
TAB-043CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-043CT-S(P)) | ELISA, Inhibion | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (pertuzumab ) | WB | |
TAB-036CT | Anti-Human HER2/neu Recombinant Antibody (6B2) | ELISA, WB | |
TAB-270CQ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-270CQ-S(P)) | WB, Inhib | Mouse scFv |
TAB-270CQ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-270CQ-F(E)) | WB, Inhib | Mouse Fab |
PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-050CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (HuA21) | ELISA, WB | Humanized antibody |
TAB-053CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-053CT-S(P)) | ELISA, Inhib, FC | Humanized scFv |
TAB-054CT-S(P) | Humanized Anti-Human HER2/neu Recombinant Antibody scFv Fragment | ELISA, Inhibition, FC | Humanized antibody |
TAB-033CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-033CT-F(E)) | ELISA, WB | Humanized Fab |
TAB-468CQ | Anti-Human ERBB2 Recombinant Antibody (Timigutuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0021-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-0021-YC) | ELISA, Neut, FC, FuncS | Human IgG |
HPAB-0010-FY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0010-FY) | ELISA, FC, ADCC | Human IgG1, κ |
HPAB-0011-FY | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-0011-FY) | Inhib, ADCC, FuncS | Mouse IgG1, κ |
HPAB-1111LY | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-1111LY) | IHC, FuncS, FC | Mouse IgG |
HPAB-1112LY | Human Anti-ERBB2 Recombinant Antibody (HPAB-1112LY) | ELISA | Chimeric (Mouse/Human) IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-CN0641 | PE-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
MHC-CN0642 | APC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
MHC-CN0643 | FITC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
MHC-CN0644 | A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
MHC-CN0645 | PE-A*02:01/Human ERBB2 (ILHDGAYSL) MHC Tetramer | FCM, IHC |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0027-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone F5); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
HPAB-S0028-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C1); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
HPAB-1855-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-1855-FY-F(E)) | ELISA | Humanized Fab |
FAMAB-0359JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone FRc13); scFv Fragment | DB, ELISA, FuncS | Human scFv |
PSBS-0070 | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (PSBS-0070) | ELISA, FuncS | Human scFv |
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