Mouse Anti-PSMC1 Recombinant Antibody (clone 26G1)
CAT#: MOB-3361z
This product is a mouse antibody that recognizes human PSMC1. It can be used in the following applications: IHC, ICC, IF, WB, FCM.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/872/2056_A_5_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_6_6.jpg
Normal Tissue
Figure 6 Skeletal muscle
Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_B_3_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100764-PSMC1/subcellular
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Specifications
- Immunogen
- Human Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PSMC1
- Species Reactivity
- Human
- Clone
- 26G1
- Applications
- IHC, ICC, IF, WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunohistochemistry Assay, Immunocytochemistry Assay, Immunofluorescence Assay, Western Blot, Flow Cytometry.
Target
- Alternative Names
- PSMC1; P56; Proteasome 26S ATPase Subunit 1; 26S Protease Regulatory Subunit 4; S4; Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1; 26S Proteasome AAA-ATPase Subunit RPT2; Proteasome 26S Subunit, ATPase, 1; P26S4; Proteasome 26S Subunit ATPase 1
- UniProt ID
- P62191
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Highly Specific in Blotting
I've utilized this antibody in our lab for Western blotting, where it has shown remarkable specificity. The clear bands for PSMC1 were beyond satisfactory and improved consistency in our results.
Reliable in Tissue Staining
In tissue preparations, this antibody provided detailed visualization of PSMC1. Its accuracy significantly improved our histological analysis.
No Cross-reactivity Observed
It's a relief to witness minimal to no cross-reactivity, which provides clarity in complex protein studies.
User-Friendly and Effective
Handling the antibody is straightforward, and the effectiveness in applications adds immense value to our projects.
Dependable for In-depth Analysis
Essential for proteasome studies, it offers dependable insights into PSMC1 actions and interactions. Incredible in advancing our research.
Q&As
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Can this antibody be used in Western blotting?
A: Yes, it exhibits excellent binding affinity in Western blots, providing clear and distinct bands when identifying the PSMC1 protein.
-
What is PSMC1, and why is it significant in research?
A: PSMC1 is a component of the 26S proteasome involved in ATP-dependent degradation of ubiquitinated proteins, crucial for maintaining cellular protein homeostasis.
-
Can this antibody be used in cell and tissue staining?
A: It is effective in both cell and tissue staining, allowing researchers to visualize the distribution and levels of PSMC1.
-
Can this antibody be conjugated with fluorophores or enzymes?
A: It can be customized with various labels for enhanced detection in fluorescent or enzymatic assays.
-
Is this antibody recommended for epitope mapping studies?
A: Certainly, its precise binding makes it a valuable tool for epitope mapping within the proteasome complex.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PSMC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1793 | Anti-PSMC1 Immunohistochemistry Kit | IHC |
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