Can this antibody be used in Western blotting?

Yes, it exhibits excellent binding affinity in Western blots, providing clear and distinct bands when identifying the PSMC1 protein.

What is PSMC1, and why is it significant in research?

PSMC1 is a component of the 26S proteasome involved in ATP-dependent degradation of ubiquitinated proteins, crucial for maintaining cellular protein homeostasis.

Can this antibody be used in cell and tissue staining?

It is effective in both cell and tissue staining, allowing researchers to visualize the distribution and levels of PSMC1.

Can this antibody be conjugated with fluorophores or enzymes?

It can be customized with various labels for enhanced detection in fluorescent or enzymatic assays.

Is this antibody recommended for epitope mapping studies?

Certainly, its precise binding makes it a valuable tool for epitope mapping within the proteasome complex.

Mouse Anti-PSMC1 Recombinant Antibody (clone 26G1) (CAT#: MOB-3361z)

This product is a mouse antibody that recognizes human PSMC1. It can be used in the following applications: IHC, ICC, IF, WB, FCM.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location and Protein Expression

Figure 1 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/872/2056_A_5_6_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_B_9_5.jpg

Figure 3 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_7_3.jpg

Figure 4 Kidney
(Collecting ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_9_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_A_6_6.jpg

Figure 6 Skeletal muscle
(Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16885/168817_B_3_6.jpg

RNA Expression

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100764-PSMC1/subcellular

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG1

Specificity
Human PSMC1

Species Reactivity
Human

Clone
26G1

Applications
IHC, ICC, IF, WB, FC

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Immunohistochemistry Assay, Immunocytochemistry Assay, Immunofluorescence Assay, Western Blot, Flow Cytometry.

Target

Alternative Names
PSMC1; P56; Proteasome 26S ATPase Subunit 1; 26S Protease Regulatory Subunit 4; S4; Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1; 26S Proteasome AAA-ATPase Subunit RPT2; Proteasome 26S Subunit, ATPase, 1; P26S4; Proteasome 26S Subunit ATPase 1

UniProt ID
P62191

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
VS-0325-XY1793	Anti-PSMC1 Immunohistochemistry Kit	IHC

Customer Reviews and Q&As

Customer Review Q&As

Highly Specific in Blotting

I've utilized this antibody in our lab for Western blotting, where it has shown remarkable specificity. The clear bands for PSMC1 were beyond satisfactory and improved consistency in our results.

Reliable in Tissue Staining

In tissue preparations, this antibody provided detailed visualization of PSMC1. Its accuracy significantly improved our histological analysis.

No Cross-reactivity Observed

It's a relief to witness minimal to no cross-reactivity, which provides clarity in complex protein studies.

User-Friendly and Effective

Handling the antibody is straightforward, and the effectiveness in applications adds immense value to our projects.

Dependable for In-depth Analysis

Essential for proteasome studies, it offers dependable insights into PSMC1 actions and interactions. Incredible in advancing our research.

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