Mouse Anti-ABL2 Recombinant Antibody (VS3-CJ958)
CAT#: VS3-CJ958
This product is a mouse antibody that recognizes human, and mouse endogenous levels of active Caspase 3 protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17106/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7849_A_5_4_selected.jpg
Normal Tissue
Figure 4 IHC staining of human caudate
Immunohistochemical staining of human caudate shows moderate positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72754/164370_B_7_7_selected.jpg
Normal Tissue
Figure 5 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17106/ihc_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7884_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7849_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7849_A_7_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7849_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1866/7849_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (hTERT-HME1, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143322-ABL2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human, Mouse endogenous levels of active Caspase 3 protein
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500), Immunohistochemistry (1:25).
Target
- Alternative Names
- ARG; ABLL
- Gene ID
- 27
- UniProt ID
- P42684
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
- Protein Refseq
- NP_001129472.1; NP_001129473.1; NP_001161708.1
- Function
- Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 (By similarity).
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Related Signaling Pathways
ErbB Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-26 | Recombinant Anti-human ABL2 Antibody | WB, Dot, FuncS | IgG |
| MRO-0019-CN | Rabbit Anti-ABL2 Recombinant Antibody (clone JF0951) | WB, FC | Rabbit IgG |
| ZG-0309F | Mouse Anti-ABL2 Recombinant Antibody (ZG-0309F) | WB, IF, ELISA | Mouse IgG |
| ZG-0310F | Mouse Anti-ABL2 Recombinant Antibody (ZG-0310F) | WB, IF, ELISA | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-26-F(E) | Recombinant Anti-human ABL2 Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| MHH-26-F(E) | Recombinant Human Anti-human ABL2 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-26-S(P) | Recombinant Anti-human ABL2 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| MHH-26-S(P) | Recombinant Human Anti-human ABL2 Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0022 | Hi-Affi™ Rabbit Anti-ABL2 Recombinant Antibody (clone DS22AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY48 | Anti-ABL2 Immunohistochemistry Kit | IHC |
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