Rabbit Anti-Acetyl-Histone H3-K23 Recombinant Antibody (clone 0725W73)
CAT#: VS-0725-FY240
This recombinant antibody, clone 0725W73, targets a synthetic acetylated peptide centered around K27 of human Histone H3. It is validated for Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Enzyme-linked Immunosorbent Assay (ELISA), and Dot Blot (DB). This antibody is a valuable tool for studying histone acetylation, a key post-translational modification controlling DNA accessibility and thus crucial for transcription regulation, DNA repair, replication, and chromosomal stability.
Specifications
- Immunogen
- Human Histone H3 (P68431): A synthetic acetylated peptide centered around K27.
Sequence: MART KQTA RKST GGKA PRKQ LATK AARK SAPA TGGV KKPH RYRP GTVA LREI RRYQ KSTE LLIR KLPF QRLV REIA QDFK TDLR FQSS AVMA LQEA CEAY.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Acetyl-Histone H3-K23
- Species Reactivity
- Human, Mouse, Rat, Other (Wide Range Predicted)
- Clone
- 0725W73
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Dot Blot
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Molecular Weight
- The calculated MW is 15 kDa, the observed MW is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 0.05% BSA, 50% glycerol, pH7.3
- Preservative
- 0.05% Proclin300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Dot Blot: 1:500-1:2000
Western Blot: 1:10000-1:120000
Immunohistochemistry-Paraffin: 1:500-1:1000
Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsImmunofluorescence
Immunocytochemistry: 1:50-1:200
Chromatin Immunoprecipitation: 2μg antibody for 5μg-10μg of Chromatin
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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