Rabbit Anti-CD3E Recombinant Antibody (clone E4T1B)
CAT#: VS-0624-FY60
This is a rabbit recombinant antibody that specifically recognizes mouse CD3E. The antigen is synthetic peptide corresponding to residues surrounding Leu50 of mouse CD3ε protein.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues surrounding Leu50 of mouse CD3e protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse CD3e
- Species Reactivity
- Mouse
- Clone
- E4T1B
- Applications
- Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
- MW
- The calculated MW is 23 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry: 1:400-1:1600
Immunohistochemistry-Paraffin: 1:100-1:400
Immunofluorescence-Frozen: 1:400-1:1600
Target
- Alternative Names
- CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3
- Gene ID
- 916
- UniProt ID
- P07766
- Long Name
- CD3e Molecule
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
- Protein Refseq
- NP_000724.1
- Function
- Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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