Mouse Anti-CDH3 Recombinant Antibody (clone TSP7) (CAT#: PABC-251)
This product is a recombinant Mouse antibody that can recognize CDH3. The epitope recognized by TSP7 is likely essential for the stability of cell adhesion mediated by P-cadherin. SP7 abolishes cell adhesion by disabling the so-called X-dimer (a kinetic adhesive intermediate), in addition to disrupting the strand-swap dimer (the final thermodynamic state).
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ELISA
Figure 1 ELISA
Binding of antibodies to P-cadherin on cells analyzed by cell ELISA. Plots of TSP5, TSP7, and TSP11 are shown in black, red, and cyan respectively. Each plot represents the mean ± SD.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
Inhib
Figure 2 Time-dependent inhibition of the cell adhesion by TSP7.
Live cell imaging using CHO cells stably expressing human P-cadherin WT-mGFP or K14E-mGFP in the absence and presence of TSP7. Images were obtained with an In Cell Analyzer 2000 instrument (GE Healthcare) with a 60 × 0.7 NA objective lens. Scale bar represents 10 μm. The arrow indicates a typical cell-cell junction region. (b) Time-dependent changes in the intensity of the dense fluorescent spots (puncta) at cell-cell boundaries. Plots for P-cadherin WT and K14E are shown as black and gray lines, respectively. Plots are the average ± SD.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
SPR
Figure 3 Kinetic parameters by SPR.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
Specifications
- Immunogen
- Whole extracellular domain constructs (EC1–EC5) of human P-cadherin expressed in CHO cells transfected with the pEF4 vector containing the constructs.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDH3
- Species Reactivity
- Human
- Clone
- TSP7
- Applications
- ELISA, Inhib, Live cell imaging
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody TSP7 has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Live cell imaging, Surface Plasmon Resonance. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Enzyme-linked Immunosorbent Assay: The antibody can be diluted into 0-100 nM for Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- CDH3; cadherin 3; type 1; P-cadherin (placental); CDHP; HJMD; PCAD; cadherin-3; calcium-dependent adhesion protein; placental;
- Gene ID
- 1001
- UniProt ID
- P22223
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0943CL | Mouse Anti-CDH3 Recombinant Antibody (TAB-0943CL) | Inhib | Mouse IgG |
| TAB-0943CL-S(P) | Mouse Anti-CDH3 Recombinant Antibody; scFv Fragment (TAB-0943CL-S(P)) | SDS-PAGE | Mouse scFv |
| TAB-0943CL-F(E) | Mouse Anti-CDH3 Recombinant Antibody; Fab Fragment (TAB-0943CL-F(E)) | SDS-PAGE | Mouse Fab |
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