Mouse Anti-CDH3 Recombinant Antibody (clone TSP7) (CAT#: PABC-251)
This product is a recombinant Mouse antibody that can recognize CDH3. The epitope recognized by TSP7 is likely essential for the stability of cell adhesion mediated by P-cadherin. SP7 abolishes cell adhesion by disabling the so-called X-dimer (a kinetic adhesive intermediate), in addition to disrupting the strand-swap dimer (the final thermodynamic state).
Specific Inquiry
ELISA
Figure 1 ELISA
Binding of antibodies to P-cadherin on cells analyzed by cell ELISA. Plots of TSP5, TSP7, and TSP11 are shown in black, red, and cyan respectively. Each plot represents the mean ± SD.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
Inhib
Figure 2 Time-dependent inhibition of the cell adhesion by TSP7.
Live cell imaging using CHO cells stably expressing human P-cadherin WT-mGFP or K14E-mGFP in the absence and presence of TSP7. Images were obtained with an In Cell Analyzer 2000 instrument (GE Healthcare) with a 60 × 0.7 NA objective lens. Scale bar represents 10 μm. The arrow indicates a typical cell-cell junction region. (b) Time-dependent changes in the intensity of the dense fluorescent spots (puncta) at cell-cell boundaries. Plots for P-cadherin WT and K14E are shown as black and gray lines, respectively. Plots are the average ± SD.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
SPR
Figure 3 Kinetic parameters by SPR.
Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.
Specifications
- Immunogen
- Whole extracellular domain constructs (EC1–EC5) of human P-cadherin expressed in CHO cells transfected with the pEF4 vector containing the constructs.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDH3
- Species Reactivity
- Human
- Clone
- TSP7
- Applications
- ELISA, Inhib, Live cell imaging
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody TSP7 has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Live cell imaging, Surface Plasmon Resonance. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Enzyme-linked Immunosorbent Assay: The antibody can be diluted into 0-100 nM for Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- CDH3; cadherin 3; type 1; P-cadherin (placental); CDHP; HJMD; PCAD; cadherin-3; calcium-dependent adhesion protein; placental;
- Gene ID
- 1001
- UniProt ID
- P22223
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0943CL | Mouse Anti-CDH3 Recombinant Antibody (TAB-0943CL) | Inhib | Mouse IgG |
| TAB-0943CL-S(P) | Mouse Anti-CDH3 Recombinant Antibody; scFv Fragment (TAB-0943CL-S(P)) | SDS-PAGE | Mouse scFv |
| TAB-0943CL-F(E) | Mouse Anti-CDH3 Recombinant Antibody; Fab Fragment (TAB-0943CL-F(E)) | SDS-PAGE | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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