Human Anti-HIV-1 Env Recombinant Antibody (clone 6B8) (CAT#: MRO-2774CQ)
Recombinant human antibody to Env. 6B8 showed significant binding only to the Gag-Env pseudovirions and not to the gp120 monomer.
Specific Inquiry
ELISA
Figure 1 MAb oligomer sensitivity.
ELISAs using the indicated antibodies were conducted side-by-side on plates coated with ConA-captured gp120 and oligomer-containing sgp140 (vAE1 supernatant).
Edinger, A. L., Ahuja, M., Sung, T., Baxter, K. C., Haggarty, B., Doms, R. W., & Hoxie, J. A. (2000). Characterization and epitope mapping of neutralizing monoclonal antibodies produced by immunization with oligomeric simian immunodeficiency virus envelope protein. Journal of virology, 74(17), 7922-7935.
Neu
Figure 2 Neutralization profiles of antibodies using heterologous SIV isolates.
Neutralization assays were conducted as described for SIVmacCP-MAC but using viruses pseudotyped with SIV Env clones SIVmac1A11.
Edinger, A. L., Ahuja, M., Sung, T., Baxter, K. C., Haggarty, B., Doms, R. W., & Hoxie, J. A. (2000). Characterization and epitope mapping of neutralizing monoclonal antibodies produced by immunization with oligomeric simian immunodeficiency virus envelope protein. Journal of virology, 74(17), 7922-7935.
Neu
Figure 3 Depicts the neutralization profiles of antibodies using heterologous SIV isolates.
Neutralization assays were conducted as described for SIVmacCP-MAC but using viruses pseudotyped with SIV Env clones SIVmac239.
Edinger, A. L., Ahuja, M., Sung, T., Baxter, K. C., Haggarty, B., Doms, R. W., & Hoxie, J. A. (2000). Characterization and epitope mapping of neutralizing monoclonal antibodies produced by immunization with oligomeric simian immunodeficiency virus envelope protein. Journal of virology, 74(17), 7922-7935.
Block
Figure 4 Ability of neutralizing MAbs to block Env-CCR5 binding.
Unpurified SIV-17E-Fr gp120 was preincubated with no antibody (NO AB) or the indicated MAbs for 30 min at RT, added to 293T cells stably expressing rhesus CCR5 or parental 293T cells, and incubated for 1 h at 37°C.
Edinger, A. L., Ahuja, M., Sung, T., Baxter, K. C., Haggarty, B., Doms, R. W., & Hoxie, J. A. (2000). Characterization and epitope mapping of neutralizing monoclonal antibodies produced by immunization with oligomeric simian immunodeficiency virus envelopprotein. Journal of virology, 74(17), 7922-7935.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- 6B8
- Applications
- ELISA, Neu
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
| PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11) | WB, ELISA, FuncS | Human IgG |
| PABL-145 | Human Anti-HIV-1 Env Recombinant Antibody (PABL-145) | ELISA, Neut, FuncS | Human IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
| PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
| PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| PFBL-145 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-145) | ELISA, Neut, FuncS | Human Fab |
MHC Tetramer for Virology
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-324 | Human Anti-HIV-1 Env Recombinant Antibody (clone 02-CC); scFv Fragment | ELISA, Neut | Human scFv |
| PSBC-325 | Human Anti-HIV-1 Env Recombinant Antibody (clone 02-K); scFv Fragment | ELISA, Neut | Human scFv |
| PSBC-326 | Human Anti-HIV-1 Env Recombinant Antibody (clone 02-CB); scFv Fragment | ELISA, Neut | Human scFv |
| PABJ-0039-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-55); scFv Fragment | ELISA, Neut | Human scFv |
| PABJ-0053-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-18); scFv Fragment | ELISA, Neut | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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