Human Anti-HIV-1 gp41 Recombinant Antibody (clone 10E8-10fH/4fL) (CAT#: MRO-4138CQ)

Figure 1 Human Anti-HIV-1 gp41 Recombinant Antibody (MRO-4138CQ) in SDS-PAGE

SDS-PAGE analysis of MRO-4138CQ in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, the different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of MRO-4138CQ was greater than 95% as determined by SDS-PAGE.

Figure 2 Human Anti-HIV-1 gp41 Recombinant Antibody (MRO-4138CQ) in DB

Dot Blot analysis of MRO-4138CQ was performed by coating with recombinant HIV1 gp41 protein (His tag).

MRO-4138CQ incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)

Figure 3 Human Anti-HIV-1 gp41 Recombinant Antibody (MRO-4138CQ) in ELISA

ELISA analysis of MRO-4138CQ was performed by coating with recombinant HIV1 gp41 protein (His tag). Then blocked with BSA and incubated with MRO-4138CQ. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.