Mouse Anti-HIV-1 Nef Recombinant Antibody (clone F14.11) (CAT#: MRO-4219CQ)
Recombinant mouse antibody to Nef(83-88). F14.11 is a nef peptide-specific Antibody (IgG2a/k) exhibiting the ability to recognize natural nef protein in either radioimmunoassay, radioimmunoprecipitation assay, or immunocytochemical analysis.
Specific Inquiry
FuncS
Figure 1 Titration curve of F14.11 on HIV-infected cell lysate.
The titration curves have been performed on microtiter plates coated with HIV-1 infected H9 cell lysates.
DE SANTIS, R. I. T. A., ANASTASI, A. M., MARCOLINI, S., VALESINI, G., PEZZELLA, M., VONESCH, N., ... & MELE, A. (1991). Production of a nef-specific monoclonal antibody by the use of a synthetic peptide. AIDS research and human retroviruses, 7(3), 315-321.
IP
Figure 2 Radioimmunoprecipitation of nefprotein by purified F14.11 (lane 1), F14.11 ascitic fluid (lane 2), or aspecific MAb (lane 3).
Lanes 1 and 2 show a protein band of
approximately 27 kD immunoprecipitated by purified F14.il
(lane 1) or F14.il ascitic fluid (lane 2) while any band is
immunoprecipitated by the purified aspecific monoclonal antibody (lane 3).
DE SANTIS, R. I. T. A., ANASTASI, A. M., MARCOLINI, S., VALESINI, G., PEZZELLA, M., VONESCH, N., ... & MELE, A. (1991). Production of a nef-specific monoclonal antibody by the use of a synthetic peptide. AIDS research and human retroviruses, 7(3), 315-321.
IF
Figure 3 Immunocytochemical staining of PBMCs from a HIV-1-infected seropositive patient with F14.11 MAb.
Typical nef+ PBMCs from seropositive subjects and their
seronegative partners, exhibit morphological detail consistent
with that of lymphocytes.
DE SANTIS, R. I. T. A., ANASTASI, A. M., MARCOLINI, S., VALESINI, G., PEZZELLA, M., VONESCH, N., ... & MELE, A. (1991). Production of a nef-specific monoclonal antibody by the use of a synthetic peptide. AIDS research and human retroviruses, 7(3), 315-321.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Recognizes HIV-1 Nef(83-88)
- Species Reactivity
- HIV-1
- Clone
- F14.11
- Applications
- IF, IP, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Nef antibody has been reported in applications of Immunofluorescence, Immunoprecipitation, Functional Assay.
Target
- Alternative Names
- HIV Nef; Human Immunodeficiency Viruse Nef; Human Immunodeficiency Viruse; HIV Nef protein; HIV Nef-protein; p27; HIV1gp9; F-protein; Negative factor
- Gene ID
- 156110
- UniProt ID
- P04601
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone F14.11"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-013 | Recombinant Anti-HIV nef VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
MHC Tetramer for Virology
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4205CQ | Human Anti-HIV-1 Nef Recombinant Antibody (clone 3B4B) | ELISA | Human IgM |
| MRO-4206CQ | Human Anti-HIV-1 Nef Recombinant Antibody (clone 4H4) | ELISA, IF, FC | Human IgG1 |
| MRO-4207CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2A3) | ELISA, WB, IHC | Mouse IgG1 |
| MRO-4208CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2EA) | ELISA, WB, IHC | Mouse IgG1 |
| MRO-4211CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 3G12) | ELISA, WB, IHC | Mouse IgG2a |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4223CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone F8); scFv Fragment | ELISA, WB | Mouse scFv |
| MRO-4224CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF1A1); scFv Fragment | ELISA | Mouse scFv |
| MRO-4225CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF2B2); scFv Fragment | ELISA | Mouse scFv |
| MRO-4226CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF3A3); scFv Fragment | ELISA | Mouse scFv |
| MRO-4227CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF8B4); scFv Fragment | ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4205CQ-F(E) | Human Anti-HIV-1 Nef Recombinant Antibody (clone 3B4B); Fab Fragment | ELISA | Human Fab |
| MRO-4206CQ-F(E) | Human Anti-HIV-1 Nef Recombinant Antibody (clone 4H4); Fab Fragment | ELISA, IF, FC | Human Fab |
| MRO-4207CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2A3); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| MRO-4208CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2EA); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| MRO-4209CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2F2); Fab Fragment | ELISA, WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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