Mouse Anti-NHP RACGAP1 Recombinant Antibody (clone OTI3A5) (CAT#: VS-1024-XY428)
Aiming at RACGAP1, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. The immunogen comprises a human recombinant protein fragment, ensuring high specificity and reliability. Suitable for applications in Western blot (WB) and immunohistochemistry (IHC), this antibody serves as a crucial reagent for investigating RACGAP1 expression and its role in cellular processes, thereby enhancing the understanding of its biological significance across various research disciplines.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39427/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39427/if_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43912/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43912/if_selected.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43912/125534_A_6_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43912/125534_A_6_6.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161800-RACGAP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161800-RACGAP1
Specifications
- Immunogen
- Human recombinant protein fragment
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- RACGAP1
- Species Reactivity
- Human, Non-human primate
- Clone
- OTI3A5
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:20
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2972 | Hi-Affi™ Recombinant Rabbit Anti-RACGAP1 Monoclonal Antibody (DS2972AB) | WB, IHC, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
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