This product is a human antibody targeting Phl p 5. This antibody is isolated from a human IgE library, which is constructed from peripheral blood B cells of an individual with timothy allergy.
Figure 1 Reactivity of human scFv (x-axis) to the Phl p 5 C-terminal domain.
Reactivity of anti-Phl p 5 antibody (clone 5) to the Phl p 5 C-terminal domain.
Levin, M., Rotthus, S., Wendel, S., Najafi, N., Källström, E., Focke‐Tejkl, M., ... & Ohlin, M. (2014). Multiple independent I g E epitopes on the highly allergenic grass pollen allergen P hl p 5. Clinical & Experimental Allergy, 44(11), 1409-1419.
Figure 2 Cross-reactivity of Phl p 5-specific recombinant IgE Fabs with natural group 5 allergens from different grasses.
Two Phl p 5-specific IgE Fabs (clone 5 and 28) and a recombinant IgG Fab with specificity for the major birch pollen allergen Bet v 1 (Co, negative control) were tested for reactivity with nitrocellulose-blotted grass pollen extracts ( Kentucky Bluegrass, P. pratense; rye, S. cereale).
Steinberger, P., Kraft, D., & Valenta, R. (1996). Construction of a combinatorial IgE library from an allergic patient isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, phl p 5. Journal of Biological Chemistry, 271(18), 10967-10972.
Figure 3 Purification of a recombinant Phl p 5-specific IgE Fab by affinity chromatography to immobilized recombinant Phl p5.
A gel containing samples of the total E. coli supernatant: Fabs (SN), the wash fraction (lane 1), and the elution fractions (lanes 2–5) of the Phl p 5 affinity column, was blotted onto nitrocellulose and Fabs were detected with a goat anti-human Fab antiserum.
Steinberger, P., Kraft, D., & Valenta, R. (1996). Construction of a combinatorial IgE library from an allergic patient isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, phl p 6. Journal of Biolocal Chemistry, 271(18), 10967-10972.
Figure 4 Influence of the preincubation of recombinant Phl p 5 with a Phl p 5-specific IgE Fab on the IgE binding of grass pollen allergic patients.
Nitrocellulose strips containing blotted recombinant Phl p 5 (duplicates) were preincubated with Phl p 5-specific IgE Fabs (clones 5) or with Bet v 1 IgG Fabs (negative control, Co) before serum IgE from two grass pollen allergic patients was applied. Bound IgE was detected with 125I-labeled anti-human IgE monoclonal antibodies.
Steinberger, P., Kraft, D., & Valenta, R. (1996). Construction of a combinatorial IgE library from an allergic patient isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, phl p 7. Journal of Biolocal Chemistry, 271(18), 10967-10972.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-770-F(E) | Recombinant Anti-Phl p 5 Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
FAMAB-0009WJ-F(E) | Human Anti-Phl p 5 Recombinant Antibody (clone 5); Fab Fragment | WB | Human Fab |
FAMAB-0010WJ-F(E) | Human Anti-Phl p 5 Recombinant Antibody (clone 14); Fab Fragment | WB | Human Fab |
FAMAB-0011WJ-F(E) | Human Anti-Phl p 5 Recombinant Antibody (clone 28); Fab Fragment | WB | Human Fab |
FAMAB-0012WJ-F(E) | Human Anti-Phl p 5 Recombinant Antibody (clone 31); Fab Fragment | WB | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-770-S(P) | Recombinant Anti-Phl p 5 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
MHH-770-S(P) | Recombinant Human Anti-Phl p 5 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
FAMAB-0010WJ-S(P) | Human Anti-Phl p 5 Recombinant Antibody (clone 14); scFv Fragment | WB | Human scFv |
FAMAB-0011WJ-S(P) | Human Anti-Phl p 5 Recombinant Antibody (clone 28); scFv Fragment | WB | Human scFv |
FAMAB-0012WJ-S(P) | Human Anti-Phl p 5 Recombinant Antibody (clone 31); scFv Fragment | WB | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0303-CN | Human Anti-Phl p 5 Recombinant Antibody (clone P5-AB10) | ELISA | Human IgG |
FAMAB-0304-CN | Human Anti-Phl p 5 Recombinant Antibody (clone P5-MB10) | ELISA | Human IgG |
FAMAB-0305-CN | Human Anti-Phl p 5 Recombinant Antibody (clone P5-AA1) | ELISA | Human IgG |
FAMAB-0306-CN | Human Anti-Phl p 5 Recombinant Antibody (clone P5-MA11) | ELISA | Human IgG |
FAMAB-1872CQ | Human Anti-Phl p 5 Recombinant Antibody (clone 4.2) | ELISA | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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