Rabbit Anti-PLCG2 Recombinant Antibody (clone R42-7F-8) (CAT#: VS3-FY2130)
We provide a rabbit recombinant monoclonal antibody that exhibits a specific binding affinity towards human PLCG2. The calculated molecular weight and estimated observed molecular weight is about 148 kDa. The R42-7F-8 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/235_H5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/235_H5_1_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/ihc_selected.jpg
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/234_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/234_H5_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/535_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/535_H5_1_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18941_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18941_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_3.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_5.jpg
Figure 8 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_6_6.jpg
Figure 9 Lymph node
(erminal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_8.jpg
(erminal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_8.jpg
Figure 10 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_5_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_5_8.jpg
Figure 11 RNA cell line category: Cell line enhanced (Daudi, HMC-1, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
Figure 11 RNA cell line category: Cell line enhanced (Daudi, HMC-1, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PLCG2
- Species Reactivity
- Human
- Clone
- R42-7F-8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 148 kDa, while the observed molecular weight stands at 148kDa.
- Related Disease
- Autoinflammation, Antibody Deficiency, And Immune Dysregulation and Familial Cold Autoinflammatory Syndrome 3
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- FCAS3; APLAID; PLC-IV; PLC-gamma-2
- Gene ID
- 5336
- UniProt ID
- D3DUL3
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphorylated on tyrosine residues by CSF1R ().
Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors.
Phosphorylation leads to increased phospholipase activity.
(P16885-PLCG2_HUMAN)
Modification sites at PhosphoSitePlus (P16885)
Modification sites at neXtProt (NX_P16885)
Glycosylation from GlyGen (P16885) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_002652.2; NP_001412678.1; NP_001412679.1; NP_001412680.1
- Function
- The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Non-small Cell Lung Cancer
Morphine Addiction
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PLCG2"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1084z | Mouse Anti-PLCG2 Recombinant Antibody (clone 19A11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| VS3-QX887 | Mouse Anti-PLCG2 Recombinant Antibody (clone 5H12-G10-C8) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-WK62 | Mouse Anti-PLCG2 Recombinant Antibody (clone E8-D9) | WB, ELISA | Mouse IgG1 |
| VS3-XY1270 | Mouse Anti-PLCG2 Recombinant Antibody (clone 1A7B8) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-XY1271 | Mouse Anti-PLCG2 Recombinant Antibody (clone 4F1E8) | ELISA, WB, FC, ICC | Mouse IgG2b |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY2130. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, FC, WB, Block
Application: ELISA, FC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, WB
Application: ELISA, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
