Rabbit Anti-PLCG2 Recombinant Antibody (clone R42-7F-8)
CAT#: VS3-FY2130
We provide a rabbit recombinant monoclonal antibody that exhibits a specific binding affinity towards human PLCG2. The calculated molecular weight and estimated observed molecular weight is about 148 kDa. The R42-7F-8 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/235_H5_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/234_H5_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/535_H5_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18941_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
erminal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_8.jpg
Normal Tissue
Figure 10 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_5_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (Daudi, HMC-1, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PLCG2
- Species Reactivity
- Human
- Clone
- R42-7F-8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 148 kDa, while the observed molecular weight stands at 148kDa.
- Related Disease
- Autoinflammation, Antibody Deficiency, And Immune Dysregulation and Familial Cold Autoinflammatory Syndrome 3
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- FCAS3; APLAID; PLC-IV; PLC-gamma-2
- Gene ID
- 5336
- UniProt ID
- D3DUL3
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphorylated on tyrosine residues by CSF1R ().
Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors.
Phosphorylation leads to increased phospholipase activity.
(P16885-PLCG2_HUMAN)
Modification sites at PhosphoSitePlus (P16885)
Modification sites at neXtProt (NX_P16885)
Glycosylation from GlyGen (P16885) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_002652.2; NP_001412678.1; NP_001412679.1; NP_001412680.1
- Function
- The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Non-small Cell Lung Cancer
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| VS3-WK62 | Mouse Anti-PLCG2 Recombinant Antibody (clone E8-D9) | WB, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2741 | Hi-Affi™ Recombinant Rabbit Anti-PLCG2 Monoclonal Antibody (DS2741AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1669 | Anti-PLCG2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5469 | Anti-Mouse PLCG2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5468 | Anti-Human PLCG2 Immunohistochemistry Kit | IHC |
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