Rabbit Anti-PRAME Recombinant Antibody (clone R08-7B6)
CAT#: VS3-FY1847
The R08-7B6 recombinant monoclonal antibody is specific for human PRAME. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This PRAME antibody was used in western blot analysis of PRAME in K562 lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68562/1854_A5_4_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_7_3.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_5_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185686-PRAME/subcellular
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WB
Figure 1 Anti-PRAME Antibody (VS3-FY1847) in WB.
The PRAME expression in K562 lysates were detected using the anti-PRAME by western blot assay.
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Specifications
- Immunogen
- Recombinant human PRAME protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PRAME
- Species Reactivity
- Human, Mouse
- Clone
- R08-7B6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 58 kDa.
- Related Disease
- Melanoma and Blastoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- MAPE; OIP4; CT130; OIP-4
- Gene ID
- 23532
- UniProt ID
- P78395
- Sequence Similarities
- Belongs to the PRAME family.
- Cellular Localization
- Cell membrane, Chromosome, Cytoplasm, Golgi apparatus, Membrane, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P78395)
Modification sites at neXtProt (NX_P78395)
- Protein Refseq
- XP_011528336.1; XP_054181336.1; XP_054185423.1; NP_001278644.1; NP_001278645.1
- Function
- Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRAME"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1701LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1701LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1702LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1702LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1703LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1703LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1704LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1704LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1705LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1705LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1701LY | Human Anti-PRAME Recombinant Antibody (HPAB-1701LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1702LY | Human Anti-PRAME Recombinant Antibody (HPAB-1702LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1703LY | Human Anti-PRAME Recombinant Antibody (HPAB-1703LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1704LY | Human Anti-PRAME Recombinant Antibody (HPAB-1704LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1705LY | Human Anti-PRAME Recombinant Antibody (HPAB-1705LY) | ELISA, WB, RIA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1703LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1703LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1704LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1704LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1705LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1705LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1706LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1706LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1707LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1707LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5648 | Anti-PRAME Immunohistochemistry Kit | IHC |
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