Rat Anti-RNA pol II CTD phospho Ser7 Recombinant Antibody (clone 0725W13)
CAT#: VS-0725-FY168
This recombinant antibody, clone 0725W13, is designed to detect the RNA Pol II CTD sequence specifically phosphorylated at serine 7, using a peptide with this modification as its immunogen. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). RNA polymerase II's carboxy-terminal domain (CTD) and its phosphorylation are essential for mRNA synthesis. This antibody provides a valuable tool for investigating RNA Pol II function and gene expression regulation.
Specifications
- Immunogen
- A peptide containing the RNA Pol II CTD sequence, specifically phosphorylated at serine 7.
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- Human RNA pol II CTD phospho Ser7
- Species Reactivity
- Human
- Clone
- 0725W13
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 240 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA polymerase II C-terminal domain phosphorylated at Serine 7
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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