Rabbit Anti-SP1 Recombinant Antibody (VS13-YC1100)
CAT#: VS13-YC1100
The Rabbit Anti-SP1 Recombinant Antibody is specific for human SP1. SP1 encodes a zinc finger transcription factor binding GC-rich motifs, influencing differentiation, growth, apoptosis, and DNA damage responses, with various post-translational modifications and isoforms. The anti-SP1 antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/100-1/500), Immunofluorescence (1/100-1/500), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/95_H4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185591-SP1
❮
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Specifications
- Immunogen
- Recombinant human SP1, aa 544-588.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SP1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Sp1 Transcription Factor; Specificity Protein 1; TSFP1;
- Gene ID
- 6667
- UniProt ID
- P08047
- Long Name
- Sp1 Transcription Factor
- Sequence Similarities
- Belongs to the Sp1 C2H2-type zinc-finger protein family.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_001238754.1; NP_003100.1; NP_612482.2
- Function
- Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.
Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
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Related Signaling Pathways
MAPK Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1233-VHH | Recombinant Anti-human SP1 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1915CT | Recombinant Mouse anti-Human SP1 Monoclonal antibody (4C8) | ELISA, FC, ICC, IF, IHC-P, WB | |
| VS3-QX1046 | Mouse Anti-SP1 Recombinant Antibody (clone 1326CT463.109.176) | WB, ICC, FC | Mouse IgG1 |
| VS3-CJ483 | Mouse Anti-SP1 Recombinant Antibody (VS3-CJ483) | WB, IF, FC | Mouse IgG1, κ |
| VS3-FY1376 | Recombinant Rabbit Anti-SP1 Antibody (clone R01-9G6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-FY1377 | Recombinant Rabbit Anti-SP1 Antibody (clone R06-9I9) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3379 | Hi-Affi™ Recombinant Rabbit Anti-SP1 Monoclonal Antibody (DS3379AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2154 | Anti-SP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6834 | Anti-Mouse SP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6833 | Anti-Human SP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6835 | Anti-Bovine SP1 Immunohistochemistry Kit | IHC |
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