Mouse Anti-TNFRSF18 Recombinant Antibody (clone 4H2D6)
CAT#: VS7-0425-WR1017
This product is a mouse IgG1 antibody that binds to human TNFRSF18. The antibody 4H2D6 recognizes specific amino acid domain of human TNFRSF18 (AA: extra 26-162). It can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human spleen
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in red pulp and cells in white pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_A_5_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_B_4_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8025/24709_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (RPMI-8226, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186891-TNFRSF18
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Specifications
- Immunogen
- Recombinant human TNFRSF18 (AA: extra 26-162) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TNFRSF18
- Species Reactivity
- Human
- Clone
- 4H2D6
- Molecule Class
- TNF-Receptor
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TNFRSF18; CD357; GITR-D; AITR; TNF Receptor Superfamily Activation-Inducible Protein; Activation-Inducible TNFR Family Receptor; Tumor Necrosis Factor Receptor Superfamily, Member 18; GITR; Glucocorticoid-Induced TNFR-Related Protein; Tumor Necrosis Facto
- Gene ID
- 8784
- UniProt ID
- Q9Y5U5
- Long Name
- Tumor necrosis factor receptor superfamily, member 18
- Cellular Localization
- Cell membrane; Membrane; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_004186.1
- Function
- Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-098CQ-F(E) | Human Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (TAB-098CQ-F(E)) | ELISA, FC, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-104CQ | Mouse Anti-TNFRSF18 Recombinant Antibody (TAB-104CQ) | ELISA, FC | Mouse IgG |
| TAB-097CQ-S(P) | Mouse Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-097CQ-S(P)) | ELISA, FC, WB | Mouse scFv |
| TAB-104CQ-S(P) | Mouse Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-104CQ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-097CQ-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (TAB-097CQ-F(E)) | ELISA, FC, WB | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-105CQ | Human Anti-TNFRSF18 Recombinant Antibody (TAB-105CQ) | ELISA, FC | Chimeric (mouse/human) IgG |
| TAB-105CQ-S(P) | Mouse Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-105CQ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-105CQ-F(E) | Human Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (TAB-105CQ-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-099CQ-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-099CQ-S(P)) | ELISA, FC | Human scFv |
| TAB-100CQ-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-100CQ-S(P)) | ELISA, FC | Human scFv |
| TAB-101CQ-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-101CQ-S(P)) | ELISA, FC | Human scFv |
| TAB-102CQ-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-102CQ-S(P)) | ELISA, FC | Human scFv |
| TAB-103CQ-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (TAB-103CQ-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| NEUT-2136CQ | Rat Anti-Tnfrsf18 Recombinant Antibody (clone DTA-1) | Block, FC, IP | Rat IgG2b |
| NEUT-2137CQ | Rat Anti-Tnfrsf18 Recombinant Antibody (NEUT-2137CQ) | Block, FC | Rat IgG2 |
| NEUT-2138CQ | Mouse Anti-Tnfrsf18 Recombinant Antibody (clone YGITR765) | FC, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2685LY-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (HPAB-2685LY-F(E)) | ELISA, IHC, ECL, WB, IP, FC, IF, RIA | Mouse Fab |
| HPAB-2686LY-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (HPAB-2686LY-F(E)) | ELISA, IHC, ECL, WB, IP, FC, IF, RIA | Mouse Fab |
| HPAB-2687LY-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (HPAB-2687LY-F(E)) | ELISA, IHC, ECL, WB, IP, FC, IF, RIA | Mouse Fab |
| HPAB-2688LY-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (HPAB-2688LY-F(E)) | ELISA, IHC, ECL, WB, IP, FC, IF, RIA | Mouse Fab |
| HPAB-2689LY-F(E) | Mouse Anti-TNFRSF18 Recombinant Antibody; Fab Fragment (HPAB-2689LY-F(E)) | ELISA, IHC, ECL, WB, IP, FC, IF, RIA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-WJ | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-0271-WJ) | ELISA, WB, Block | Humanized IgG |
| HPAB-0272-WJ | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-0272-WJ) | ELISA, WB, FC, Agonist | Humanized IgG |
| HPAB-0275-WJ | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-0275-WJ) | ELISA, WB, Block | Humanized IgG |
| HPAB-0276-WJ | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-0276-WJ) | ELISA, WB, Block | Humanized IgG |
| HPAB-0278-WJ | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-0278-WJ) | ELISA, WB, Block | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0272-YC-S(P) | Human Anti-TNFRSF18 Recombinant Antibody (HPAB-N0272-YC-S(P)); scFv Fragment | Block | Human scFv |
| HPAB-N0273-YC-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (HPAB-N0273-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-N0274-YC-S(P) | Human Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (HPAB-N0274-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-0440-WJ-S(P) | Mouse Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (HPAB-0440-WJ-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0445-WJ-S(P) | Mouse Anti-TNFRSF18 Recombinant Antibody; scFv Fragment (HPAB-0445-WJ-S(P)) | ELISA, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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