Rabbit Anti-TOP1 Recombinant Antibody (VS13-YC1174) (CAT#: VS13-YC1174)

• Immunogen
• Recombinant human Topoisomerase I.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat TOP1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC-Fr, IHC-P, ICC, IF

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.

• Preservative
• 0.01% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunohistochemistry (IHC): 1/50-1/100
  Immunofluorescence (IF): 1/50-1/200

• Alternative Names
• DNA Topoisomerase I; Topoisomerase (DNA) I; EC 5.99.1.2; Type I DNA Topoisomerase; DNA Topoisomerase 1; TOPI;

• Gene ID
• 7150

• UniProt ID
• P11387

• Long Name
• DNA Topoisomerase I

• Sequence Similarities
• Belongs to the type IB topoisomerase family.

• Cellular Localization
• Nucleus

• Protein Refseq
• NP_003277.1

• Function
• Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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