Rabbit Anti-TOP1 Recombinant Antibody (VS3-FY2904)
CAT#: VS3-FY2904
The anti-TOP1 antibody is a rabbit recombinant monoclonal antibody. It has been specifically designed to target the human TOP1 protein. It cross-reacts with mouse, rat species. This monoclonal antibody proves valuable in disease research related to Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Neutropenia. The antibody can be utilized for detection of target protein by Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/154_H7_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows general nuclear positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_3_2_selected.jpg
Subcellular Location
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9058/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/198_H7_1_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/152_H7_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_8_5.jpg
Normal Tissue
Figure 9 Urinary bladder
Urothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_5_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198900-TOP1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TOP1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Neutropenia
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- DNA Topoisomerase I; Topoisomerase (DNA) I; EC 5.99.1.2; Type I DNA Topoisomerase; DNA Topoisomerase 1; TOPI
- Gene ID
- 7150
- UniProt ID
- P11387
- Sequence Similarities
- Belongs to the type IB topoisomerase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
- Protein Refseq
- NP_003277.1
- Function
- Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TOP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3638 | Rabbit Anti-TOP1 Recombinant Antibody (clone DS3638AB) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0004JF | Chicken Anti-TOP1 Recombinant Antibody (clone 5C2) | WB, ELISA, IP, FC | |
| VS3-QX1142 | Mouse Anti-TOP1 Recombinant Antibody (clone 1291CT875.142.166) | WB, FC | Mouse IgG1, κ |
| VS3-CJ591 | Mouse Anti-TOP1 Recombinant Antibody (VS3-CJ591) | WB, FC | Mouse IgG1, κ |
| VS3-CJ592 | Rabbit Anti-TOP1 Recombinant Antibody (VS3-CJ592) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1470 | Recombinant Mouse Anti-TOP1 Antibody (clone 3D4B1) | ELISA, WB, ICC, FC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0004JF-F(E) | Chicken Anti-TOP1 Recombinant Antibody (clone 5C2); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0004JF-S(P) | Chicken Anti-TOP1 Recombinant Antibody (clone 5C2); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7424 | Anti-TOP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7425 | Anti-Mouse TOP1 Immunohistochemistry Kit | IHC |
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