In the realm of modern antibody conjugation technology, Creative Biolabs is proud to be at the forefront of this evolution with our advanced antibody-anionic liposome production platform. Our expertise lies in the seamless integration of monoclonal antibodies with potent anionic liposome agents.

Services

Anionic liposomes are lipid-based vesicles that carry a negative charge, typically composed of phospholipids such as lecithin or other lipids with anionic groups. They hold significant value in biomedical and drug delivery applications.

Creative Biolabs can prepare liposomes using various anionic lipids. We typically combine anionic lipids with other inorganic or organic components to enhance their stability and targeting capabilities. Our anionic liposome products are widely used in drug delivery and vaccine development due to their excellent biocompatibility and biodegradability.

The most common anionic lipids in our labs include:

Phosphatidylglycerol (PG): This lipid is commonly found in biological membranes and liposomes, where it forms an anionic structure.

Phosphatidylserine (PS): This lipid is abundant on the inner side of cell membranes and exhibits strong biocompatibility and biological activity, making it a frequent choice for drug delivery systems.

Cholesterol sulfate: This lipid can interact with other lipids to aid in the formation of anionic liposomes.

Sulfatide: This class of lipids contains sulfate groups and typically exhibits anionic properties.

Fig.1 The Common Anionic Lipids Types.^1,3

Procedures

Creative Biolabs offers antibody-anionic liposome conjugation services to assist clients in preparing drug carriers with specific targeting properties, thereby enhancing drug bioavailability and therapeutic effectiveness. Meanwhile, our laboratory specializes in the preparation and functionalization of liposomes and can also customize suitable conjugation solutions to meet your needs.

I. Selection and Preparation of Phospholipids

1. Choosing Suitable Phospholipids: Select phospholipids with anionic characteristics, such as distearoylphosphatidic acid (DSPC) or lecithin.

2. Dissolving Phospholipids: Dissolve the selected phospholipids in an appropriate organic solvent, such as chloroform or ethanol, to create a solution.

II. Preparation of Liposomes

1. Film Deposition: Evenly coat the phospholipid solution onto the walls of a dry vessel and evaporate the organic solvent to form a thin film.

2. Rehydrating the Film: Add an appropriate volume of buffer (PBS) to the film and rehydrate it using methods such as sonication, vortex mixing, or high-pressure homogenization to form liposomes.

3. Filtration and Purification: Remove unencapsulated components through filtration or dialysis to obtain purified anionic liposomes.

III. Antibody Conjugation

1. Selecting Antibodies: Choose monoclonal or polyclonal antibodies with specific targeting capabilities.

2. Activating Antibodies: Activate the antibodies using chemical reagents (EDC/NHS) to enhance their conjugation capacity with the liposomes.

3. Conjugation Reaction: Mix the activated antibodies with the anionic liposomes and incubate under suitable conditions, such as at 37°C for a specified time.

4. Removing Free Antibodies: Eliminate unbound free antibodies through dialysis or ultrafiltration to obtain the conjugated liposomes.

IV. Characterization and Analysis

1. Particle Size and Distribution: Measure the particle size and distribution of liposomes using dynamic light scattering (DLS) techniques.

2. Encapsulation Efficiency: Determine the concentration of the encapsulated substances in the liposomes using HPLC or UV-Vis spectroscopy, and calculate the encapsulation efficiency.

3. Conjugation Efficiency: Validate the conjugation efficiency of the antibodies using ELISA or other immunoassays.

4. Stability Testing: Assess the stability of liposomes under various conditions to determine their optimal storage conditions.

Fig.2 SLN Imaging by The Optimized Anionic Liposomes.^2,3

Advantages

At Creative Biolabs, the preparation of negatively charged liposomes involves optimizing various aspects such as lipid selection, component ratios, and coupling methods to ensure that the final product's performance meets specific therapeutic goals. In previous projects, our negatively charged liposome antibody conjugates have demonstrated several notable advantages:

• possess strong biocompatibility due to their negatively charged surface;
• can bind with neutral drugs and enhance drug delivery efficiency;
• can traverse cell membranes, further facilitating the release and accumulation of drugs within cells;
• can specifically deliver drugs or therapeutic agents to target cells, minimizing damage to normal cells;
• can improve the stability, controlled release, and targeting capabilities of antibody drugs;
• can flexibly adjust the composition of the anionic-liposome and the method of antibody coupling.

Creative Biolabs offers a customizable platform that enables our worldwide customers to develop antibody-anionic liposomes. Our skilled team works closely with clients to understand their needs, helping to design and optimize conjugates that align with your project objectives. You can easily place your order on our website or contact us by sending an email.

For research use only. Not intended for any clinical use.