Magic™ Auto-inducible Bacillus subtilis Expression Service

The Magic™ Auto-inducible Bacillus subtilis Expression Service of Creative Biolabs has adopted a novel expression technique of Bacillus subtilis for heterologous proteins (antibody, vaccine, functional peptide, etc.). Meanwhile, our creative platform has greatly optimized the original system to achieve an increased product yield and a superior bioactivity with the lowest expenditure. Winging up with our reliable platform, researchers will acquire the most valuable bio-products in the world.

The Introduction and Application of Auto-inducible Bacillus subtilis Expression

Although the B. subtilis manifests so many advantages as a biopharmaceutical producing host, the conventional standard B. subtilis system still suffers from the expensive inducer cost by using the positively regulated manP promoter of the mannose operon. In addition, other conventional induction systems also suffer a similar inducer drawback, such as the sugars, isopropyl-β-D-thiogalactopyranoside (IPTG), temperature shifts, acid shock, or ethanol, etc.

To addressing this critical technique bottleneck, the creative researchers were inspired by the existence of a few natural auto-inducible B. subtilis systems (the pst operon system, the gsiB system, and the lysine riboswitch system). In addition to the clear mechanisms of sugar activation and repression in the metabolism of B. subtilis, these pioneers created an incredible auto-inducible mechanism to fulfill the instantly growing demand for heterologous pharmaceuticals. Particularly, the auto-inducible system manipulates the mannose operon by deletion of the manP gene, which leads to a constitutive activation of the mannose operon without mannose. For instance, the TQ356 stands for a common strain of the auto-inducible system with an improved yield. Moreover, the high-cell-density culture technique furtherly improved the expression and accumulation efficiency.

Advantages of Magic™ Auto-inducible Bacillus subtilis Expression Service

1. Adopt auto-inducible strategy to bypass the inducer problem.
2. Combine high-cell-density culture technique to elevate yield of 5-folds.
3. Multiple excellent strains and vector systems for regular and toxic products via intracellular accumulation or secretion expression.
4. Optimal cultivation and induction at Creative Biolabs with the whole range of scales from 5 mL to 1000 L per batch for the conventional amylases, proteases, lipases, riboflavin, etc., and multiple antibodies (conventional antibodies and novel intrabodies), vaccines, functional peptides, and other biopharmaceuticals, with reliable and superior yields and bioactivities.
5. One-stop service: from initial design and optimization all the way down to purification and further modification with well recorded data and organized report.

The Magic™ Auto-inducible Bacillus subtilis Expression Service of Creative Biolabs is dedicated to improving the yield greatly with a sophisticated combination of the novel auto-inducible system and high-cell-density fermentation. In addition to the diligent optimizations and professional experiences, our labs guarantee a series of the most professional services

Reference

1. Wenzel, Marian, et al. "Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation." Applied and environmental microbiology 77.18 (2011): 6419-6425.

For research use only. Not intended for any clinical use.