Magic™ Bacillus subtilis Expression Service

The most diligent scientists in Creative Biolabs are dedicated to developing the most reliable and advanced Bacillus subtilis expression service in the world. During decades’ accumulation, our Magic™ Bacillus subtilis Expression Service has been built up for the exponentially growing demanding of biopharmaceutical products, such as recombinant functional proteins, research or therapeutic antibodies, and universal or specific vaccines. Winging up with our professional help, researchers will achieve numerous superior bioproducts with a magically high yield and bioactivity.

The Introduction and Application of Bacillus subtilis Expression

The Bacillus subtilis is a Gram-positive bacterium isolated from the soil originally. Although it has been applied in agriculture and traditional food technology for centuries, the great scientific advancement later revealed this incredible microorganism as a rod-shaped facultative anaerobe with a general length of 4-10 μm and 0.25-1.0 μm in diameter, which forms approximate 4.6 f L cell volume at stationary phase. As one of the best-studied model bacteria, the Bacillus subtilis manifests as an increasingly popular bacterial champion for more and more applications for heterologous protein expression among several widely used prokaryotic organisms to produce multiple enzymes and other functional protein productions, no matter in the basic research area or commercial industrial realm. Noticeable, the current application of B. subtilis in the industrial realm accounts for over 60 % of the commercial enzymes production for the following attractive characteristics:

i. Excellent amenability for the small- and large-scale fermentation;

ii. Complete basic characterization of multiple molecular mechanisms, such as its completely sequenced genome, transcription, translation, protein folding, secretion mechanism, and metabolic pathways;

iii. Non-pathogenic and endotoxin-free microorganism labeled with a Generally Regarded as Safe (GRAS) status by the U.S. Food and Drug Administration;

iv. Capable of expressing heterologous proteins (antibody, vaccine, functional peptide, etc.) without significant bias in codon usage and secreting them into extracellular culture media with high yield.

Advantages of Magic™ Bacillus subtilis Expression Service

1. Almost 96 % success rate for nearly a hundred of proteins with excellent solubility.
2. Multiple excellent strains for toxic products and secretion expression.
3. Optimal cultivation and induction at Creative Biolabs with the whole range of scales from 5 mL to 1000 L per batch.
4. One-stop service: from initial design and optimization all the way down to purification and further modification with well recorded data and organized report.

Since the Bacillus subtilis manifests so many advantages depicted above, our scientists at Creative Biolabs have focused on this incredible organism for decades. Particularly, our team has overcome the intrinsic drawbacks of this prokaryotic expression technology on the one hand. For instance, we have acquired several protease-deficient strains for the problematic existence of extracellular proteases, which would specifically degrade heterologous products. As for another major stability problem of the expression vector, further research and our efforts have completely bypassed this obstacle with the mode of replication and created multiple superior stable expression vectors derived from the natural plasmid. Thus, our service provides multiple related services for either intracellular expression or secretion production with the most professional optimization to achieve superior yield, including the superior E. coli - B. subtilis shuttle vectors with sophisticated controllable elements and corresponding derivatives with various tags (8×His, Strep, c-Myc, etc.), hundreds of SP sequences, almost every proprietary B. subtilis strains or derivatives, creative high cell density fermentation procedures, and varied induction techniques (Sugar Inducible and Auto Inducible).

The Magic™ Bacillus subtilis Expression Service of Creative Biolabs offers the first-rate services of biopharmaceutical proteins production in the world. Don’t hesitate, contact us now.

References

1. Allen, C. S., et al. "Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique." Applied microbiology and biotechnology 98.2 (2014): 855-862.
2. Jannière, Laurent, Claude Bruand, and S. Dusko Ehrlich. "Structurally stable Bacillus subtilis cloning vectors." Gene 87.1 (1990): 53-61.
3. Luan, Chao, et al. "Expressing antimicrobial peptide cathelicidin-BF in Bacillus subtilis using SUMO technology." Applied microbiology and biotechnology 98.8 (2014): 3651-3658.
4. Titok, M. A., et al. "Bacillus subtilis soil isolates: plasmid replicon analysis and construction of a new theta-replicating vector." Plasmid 49.1 (2003): 53-62.
5. van Dijl, JanMaarten, and Michael Hecker. "Bacillus subtilis: from soil bacterium to super-secreting cell factory." Microbial cell factories 12.1 (2013): 3.

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Platform:

• Magic™ Escherichia coli Expression Service

For research use only. Not intended for any clinical use.