Recombinant Rabbit Anti-ALPL Antibody (clone R08-6H0) (CAT#: VS3-FY70)

Name: Recombinant Rabbit Anti-ALPL Antibody (clone R08-6H0)
SKU: VS3-FY70
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human and rat ALPL protein. It can detect a band of approximately 57 kDa (observedd molecular weight: 75 kDa).

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-ALPL Antibody (clone R08-6H0) in WB.

Figure 1. Recombinant Rabbit Anti-ALPL Antibody (clone R08-6H0) in WB.

Western blot analysis of alkaline phosphatase in Hela, C6 lysates using alkaline phosphatase antibody.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8765/45_H3_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20829/if_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20829/47120_B_8_5.jpg

Figure 4 Adrenal gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8765/23561_B_5_5.jpg

Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20829/47120_A_7_3.jpg

Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20829/47120_A_7_5.jpg

Figure 7 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20829/47120_A_5_6.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced.
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162551-ALPL

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human Alkaline Phosphatase, Tissue Non-Specific

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Rat ALPL

Species Reactivity
Human, Rat

Clone
R08-6H0

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 57 kDa; Observed MW: 75 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Alkaline Phosphatase, Liver/Bone/Kidney; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; EC 3.1.3.1; AP-TNAP; TNSALP; Alkaline Phosphatase, Tissue-Nonspecific Isozyme

Gene ID
249

UniProt ID
P05186

Cellular Localization
Cell membrane, Membrane, Mitochondrion

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ALPL"

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CAT	Product Name	Application	Type
MOB-2224z	Mouse Anti-ALPL Recombinant Antibody (clone 37A12)	FC, ICC, IF, IHC	Mouse IgG1, κ
MOB-0477MZ	Mouse Anti-ALPL Recombinant Antibody (clone Q5B20F5)	ELISA, IP, RIA	Mouse IgG1
VS3-QX51	Mouse Anti-ALPL Recombinant Antibody (clone 7G5-D2-D7)	WB, IHC, ICC, FC, ELISA	Mouse IgG1
VS3-CJ404	Rabbit Anti-ALPL Recombinant Antibody (VS3-CJ404)	WB, ELISA	Rabbit IgG
VS7-HM91	Mouse Anti-ALPL Recombinant Antibody (clone 2F4)	WB, IHC, FCM	Mouse IgG1

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