Recombinant Rabbit Anti-HSPE1 Antibody (clone R02-3K6)
CAT#: VS3-FY714
This product is a recombinant rabbit antibody that recognizes HSPE1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R02-3K6 is specific for human, mouse, rat HSPE1. The antigen is human cpn10 synthetic peptide.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-HSPE1 Antibody (clone R02-3K6) in WB.
Western Blot analysis of Cpn10 in K562, Hela lysates using Cpn10 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-HSPE1 Antibody (clone R02-3K6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cpn10 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Normal Tissue
Figure 1 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_A_8_8.jpg
Normal Tissue
Figure 2 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_B_4_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48272/133112_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115541-HSPE1
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Specifications
- Immunogen
- Human Cpn10 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat HSPE1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-3K6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 11 kDa; Observed MW: 11 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSPE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2672z | Mouse Anti-HSPE1 Recombinant Antibody (clone 42A3) | WB, ELISA | Mouse IgG1 |
| MOB-0723MZ | Recombinant Mouse Anti-Human HSPE1 Antibody (clone M1.4) | WB | Mouse antibody |
| MRO-0384-CN | Rabbit Anti-HSPE1 Recombinant Antibody (clone JG82-34) | WB, IHC, IP | Rabbit IgG |
| VS13-YC559 | Rabbit Anti-HSPE1 Recombinant Antibody (VS13-YC559) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1705 | Rabbit Anti-HSPE1 Recombinant Antibody (clone DS1705AB) | FC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3346 | Anti-HSPE1 Immunohistochemistry Kit | IHC |
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