Recombinant Mouse Anti-PDCD6IP Antibody (clone 1E7E2) (CAT#: VS4-CJ68)

• Immunogen
• Recombinant Human Programmed cell death 6-interacting protein

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human PDCD6IP

• Species Reactivity
• Human

• Clone
• 1E7E2

• Applications
• ELISA, WB

• Conjugate
• Unconjugated

• Purification
• Protein G purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.01M PBS, pH 7.4.

• Preservative
• 0.03% Proclin 300

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000).

• Alternative Names
• AIP1; ALIX; HP95; DRIP4; MCPH29

• Gene ID
• 10015

• UniProt ID
• Q8WUM4

• Long Name
• Programmed cell death 6 interacting protein

• Cellular Localization
• Cell junction, Cytoplasm, Cytoskeleton, Secreted

• Post Translation Modifications
• May be phosphorylated on tyrosine residues by activated PDGFRB.

• Protein Refseq
• NP_001155901.1; NP_001243121.1; NP_037506.2

• Function
• Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459).
  The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548).
  Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129).
  May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413).
  By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).
  (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS