Recombinant Rabbit Anti-PRKCB Antibody (clone R05-3F4)
CAT#: VS3-FY1192
This product is a recombinant rabbit antibody that recognizes PRKCB. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R05-3F4 is specific for human, rat PRKCB. The antigen is synthetic peptide of human pkc beta 2.
Tested Data
ICC
Figure 1. Recombinant Rabbit Anti-PRKCB Antibody (clone R05-3F4) in ICC.
Immunocytochemical analysis of PKC beta 1 (green) in K562 using PKC beta 1 antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-PRKCB Antibody (clone R05-3F4) in WB.
Western Blot analysis of PKC beta 1 in K562 lysates using PKC beta 1 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human PKC beta 2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat PRKCB
- Species Reactivity
- Human, Rat
- Clone
- R05-3F4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 77 kDa; Observed MW: 77 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRKCB; PKCB; PRKCB1; Protein kinase C beta type; PKC-B; PKC-beta
- Gene ID
- 5579
- UniProt ID
- P05771
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).
- Protein Refseq
- NP_002729.2; NP_997700.1
- Function
- Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012).
Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A (PubMed:20228790).
In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed:25982116).
Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).
Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed:19176525).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PRKCB"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2276z | Mouse Anti-PRKCB Recombinant Antibody (clone 43G12) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MRO-1236-CN | Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (JM43-26) | WB, IHC, FC | Rabbit IgG |
| MRO-1237-CN | Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (CBACN-451) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0413U | Rabbit Anti-PRKCB Recombinant Antibody (clone N20-N), FITC | FC | Rabbit IgG |
| ZG-0414U | Rabbit Anti-PRKCB Recombinant Antibody (clone Q19-V), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2854 | Hi-Affi™ Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (DS2854AB) | IHC-P, WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY217 | AbPlus™ Anti-PRKCB Magnetic Beads (CBACN-451) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC936 | CytoStream™ Rabbit Anti-PRKCB Recombinant Antibody (VS13-YC936) | WB, IHC-P, FC | Rabbit IgG |
| VS13-YC937 | CytoStream™ Rabbit Anti-PRKCB Recombinant Antibody (VS13-YC937) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1750 | Anti-PRKCB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5691 | Anti-Mouse PRKCB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5690 | Anti-Human PRKCB Immunohistochemistry Kit | IHC |
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