Anti-CD20 Antibody-Cytokine Fusion Protein, IgG (DI-Leu16)-IL2 (CAT#: ACFP-SH134)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD20 IgG to IL2. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD20 as well as the biological activity of IL2. In-vitro studies of DI-Leu16-IL2 have demonstrated CD20 targeting, IL2 bioactivities, and antibody-dependent cell-mediated cytotoxicity (ADCC) functions. In a SCID mouse model, DI-Leu16-IL2 showed superior anti-lymphoma effects over rituximab or rituximab combined with systemic IL2. This immunocytokine was designed for treating Non-Hodgkin Lymphoma.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
Figure 4 Colon
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
Figure 5 Bone marrow
(Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
(Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
Figure 6 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
Figure 7 RNA cell line category: Group enriched (Daudi, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
Specifications
- Target
- CD20
- Molecule Class
- L Kappa; IgG1-IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- DI-Leu16
- Related Disease
- Non-Hodgkin Lymphoma
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- MS4A1; membrane-spanning 4-domains; subfamily A; member 1; B1; S7; Bp35; CD20; CVID5; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
- Gene ID
- 931
- UniProt ID
- A0A024R507
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-016 | Anti-Human CD20 Recombinant Antibody (Rituximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-1633CL | Anti-Human MS4A1 Recombinant Antibody (ch1F5) | FC, Apop, CDC, ADCC, Inhib | Chimeric antibody (mouse/human) |
| TAB-1634CL | Anti-Human MS4A1 Recombinant Antibody (ch1F5-DXL) | FC, Apop, CDC, ADCC, Inhib | Chimeric antibody (mouse/human) |
| TAB-1656CL | Anti-Human MS4A1 Recombinant Antibody (ch2B8) | FC, CMCD, ADCC, Activ, Inhib, Depletion | Chimeric antibody (mouse/human) |
| PABX-026 | Recombinant Human Anti-CD20 Antibody (C2H7) | FuncS | IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Enhanced B-cell Targeting
The combination of the anti-CD20 antibody with IL2 in this fusion protein significantly improved our results in B-cell targeting studies. It's a powerful tool for oncology research.
Robust in In Vivo Studies
We saw substantial tumor regression in our murine models using this fusion protein. The IL2 component proves essential for an enhanced immune response.
Perfect for In-Depth Analysis
Using this fusion protein in flow cytometry allowed deep insights into cell surface interactions and immune cell activation. Highly recommended!
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For Research Use Only. Not For Clinical Use.
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