Anti-CD20 Antibody-Cytokine Fusion Protein, IgG (DI-Leu16)-IL2
CAT#: ACFP-SH134
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD20 IgG to IL2. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD20 as well as the biological activity of IL2. In-vitro studies of DI-Leu16-IL2 have demonstrated CD20 targeting, IL2 bioactivities, and antibody-dependent cell-mediated cytotoxicity (ADCC) functions. In a SCID mouse model, DI-Leu16-IL2 showed superior anti-lymphoma effects over rituximab or rituximab combined with systemic IL2. This immunocytokine was designed for treating Non-Hodgkin Lymphoma.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
❮
❯
Specifications
- Target
- CD20
- Molecule Class
- L Kappa; IgG1-IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- DI-Leu16
- Related Disease
- Non-Hodgkin Lymphoma
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- MS4A1; membrane-spanning 4-domains; subfamily A; member 1; B1; S7; Bp35; CD20; CVID5; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
- Gene ID
- 931
- UniProt ID
- A0A024R507
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ACFP-SH134. Click the button above to contact us or submit your feedback about this product.
Enhanced B-cell Targeting
The combination of the anti-CD20 antibody with IL2 in this fusion protein significantly improved our results in B-cell targeting studies. It's a powerful tool for oncology research.
Robust in In Vivo Studies
We saw substantial tumor regression in our murine models using this fusion protein. The IL2 component proves essential for an enhanced immune response.
Perfect for In-Depth Analysis
Using this fusion protein in flow cytometry allowed deep insights into cell surface interactions and immune cell activation. Highly recommended!
Q&As
-
How does the fusion of IL2 to the Anti-CD20 antibody enhance therapeutic efficacy?
A: The IL2 component activates cytotoxic T-cells and natural killer cells, thereby amplifying the immune response against CD20-positive cancer cells targeted by the antibody.
-
Are there any specific secondary reagents recommended for use with this fusion protein?
A: Secondary antibodies specific to human IgG or detection reagents compatible with IL2 can be used for detailed analysis, depending on the experimental setup.
-
What are the recommended concentrations for in vitro assays using this fusion protein?
A: The recommended concentrations vary based on the assay type but starting ranges from 0.1 to 10 µg/mL for cell-based assays. It is advisable to optimize the concentration for each specific application.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "MS4A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-028 | Anti-Human CD20 Recombinant Antibody (TAB-028) | FC, IP, ELISA, Neut, FuncS, IF, IHC | IgG1 - kappa |
| TAB-1648CL-S(P) | Anti-MS4A1 Recombinant Antibody scFv Fragment (MEDI-552) | Inhib | |
| TAB-1654CL-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1654CL-S(P)) | FC, Apop | Human scFv |
| TAB-1655CL-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1655CL-S(P)) | ELISA | Human scFv |
| TAB-1674CL-S(P) | Human Anti-MS4A1 Recombinant Antibody scFv Fragment (TAB-1674CL-S(P)) | Cyt, ELISA, FuncS, Inhib |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-133 | Anti-Human CD20 Recombinant Antibody (Afutuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | IgG3 - kappa |
| TAB-1635CL | Anti-Human MS4A1 Recombinant Antibody (BVX20) | Inhib | Humanized antibody |
| TAB-1636CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1636CL) | ADCC, CDC | Humanized IgG |
| TAB-1637CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1637CL) | ADCC, CDC | Humanized IgG |
| TAB-1638CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1638CL) | ADCC, CDC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-143 | Human Anti-MS4A1 Recombinant Antibody (clone KM3575) | WB | Chimeric (mouse/human) IgG1, κ |
| MOB-0279MZ | Mouse Anti-MS4A1 Recombinant Antibody (clone M27 (1, 4).) | IHC | Mouse IgG2a |
| FAMAB-0144-CN | Human Anti-MS4A1 Recombinant Antibody (clone C2B8) | WB, ELISA | Chimeric (mouse/human) IgG1 |
| HPAB-0261CQ | Mouse Anti-MS4A1 Recombinant Antibody (clone CBM502) | ELISA, FC, FuncS | Mouse IgG2a |
| HPAB-0262CQ | Human Anti-MS4A1 Recombinant Antibody (HPAB-0262CQ) | ELISA, FC, Apop, ADCC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1633CL-F(E) | Anti-Human MS4A1 Recombinant Antibody Fab Fragment (ch1F5) | FC | Chimeric antibody (mouse/human) |
| TAB-1634CL-F(E) | Anti-Human MS4A1 Recombinant Antibody Fab Fragment (ch1F5-DXL) | FC, Apop, CDC, ADCC, Inhib | Chimeric antibody (mouse/human) |
| TAB-1656CL-F(E) | Anti-Human MS4A1 Recombinant Antibody Fab Fragment (ch2B8) | FC, CMCD, ADCC, Activ, Inhib, Depletion | Chimeric antibody (mouse/human) |
| TAB-1665CL-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1665CL-F(E)) | CDC | Chimeric (mouse/human) Fab |
| TAB-1666CL-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1666CL-F(E)) | CDC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-059LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
| Gly-063LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-097LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
| Gly-115LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-138LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-063LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-134LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-135LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-136LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-158LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-142LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Hyper-galactosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-155LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-171LC | Recombinant Anti-Human MS4A1 Antibody (Non-glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC066 | PE-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC067 | APC-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC068 | BV421-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC4347 | PE-B*07:02/Human MS4A1 (RPKSNIVLL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1751CQ | Mouse Anti-MS4A1 Recombinant Antibody (clone CBLS-211) | Neut, FuncS | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2295 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone DS2295AB) | IHC-Fr, IHC-P | Rabbit IgG |
| MOR-4024 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone SI88DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0241CQ-F(E) | Mouse Anti-MS4A1 Recombinant Antibody (clone 2H7); Fab Fragment | ELISA, FC, IHC, IP | Mouse Fab |
| HPAB-0138-WJ-F(E) | Human Anti-MS4A1 Recombinant Antibody (clone L4H7); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0139-WJ-F(E) | Human Anti-MS4A1 Recombinant Antibody (clone L4H5); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1321-FY-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-1321-FY-S(P)) | FC, ELISA | Human scFv |
| HPAB-1322-FY-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-1322-FY-S(P)) | FC, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0144-CN-S(P) | Human Anti-MS4A1 Recombinant Antibody (clone C2B8); scFv Fragment | WB, ELISA | Mouse scFv |
| HPAB-M0452-YC-S(P) | Canine Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-M0452-YC-S(P)) | FC, IHC | Canine scFv |
| HPAB-M0453-YC-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-M0453-YC-S(P)) | FC, IHC | Humanized scFv |
| HPAB-1321-FY-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1321-FY-F(E)) | FC, ELISA | Human Fab |
| HPAB-1322-FY-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1322-FY-F(E)) | FC, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0704-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0704-YC) | ELISA, FuncS | Camelid VHH |
| HPAB-M0705-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0705-YC) | ELISA | Camelid VHH |
| HPAB-M0706-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0706-YC) | ELISA | Camelid VHH |
| HPAB-M0707-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0707-YC) | ELISA | Camelid VHH |
| HPAB-M0708-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0708-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-772 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-772) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-016 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-016) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-028 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-028) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-207 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-207) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-771 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-771) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY188 | AbPlus™ Anti-MS4A1 Magnetic Beads (VS-0424-XY188) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC138 | Human Anti-MS4A1 Recombinant Antibody Hexamer (VS-0924-YC138), CDC Enhanced | FC, CDC, IP | Antibody hexamer |
| VS-0924-YC139 | Mouse Anti-MS4A1 Recombinant Antibody Hexamer (VS-0924-YC139), CDC Enhanced | FC, Activ, Agonist, IF, WB, Cyt, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY85 | Mouse Anti-NHP MS4A1 Recombinant Antibody (VS-1024-XY85) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY30 | Human Anti-MS4A1 (clone RW005) scFv-Fc Chimera | FuncS | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY168 | CytoStream™ Rat Anti-MS4A1 Recombinant Antibody (clone GOT214A) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC513 | Recombinant Anti-MS4A1 Vesicular Antibody, EV Displayed (VS-0425-YC513) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4510 | Anti-MS4A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4511 | Anti-Mouse MS4A1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC129 | Recombinant Anti-MS4A1 (Bin I x Bin II) Biparatopic Antibody, Tandem scFv | ELISA, FC | Tandem scFv |
| VS-0525-YC222 | Recombinant Anti-MS4A1 Biparatopic Antibody, Tandem scFv | ELISA, IF, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: Neut, FC, IHC-Fr, IP, BA
Application: ELISA, IHC, FC, IP, IF, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
