Human Anti-CD4 Recombinant Antibody (clone Hu5A8) (CAT#: PABL-427)

Recombinant Humanized Antibody (Hu5A8) is capable of binding to CD4. This is a humanized IgG4 monoclonal antibody (mAb) derived from a mouse antibody, 5A8, which binds D2 of CD4 and blocks HIV-1 infection in vitro.


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  • Published Data
  • Gene Expression
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ELISA

Figure 1 Design and characterization of functional BiIAs.

Figure 1 Design and characterization of functional BiIAs.

ELISA binding profiles of BiIA-DG and BiIA-SG to human sCD4, compared with IA-PGT128, IA-Hu5A8, and VRC01-IgG. Data represent mean ± SD with duplicates.

Wu, X., Guo, J., Niu, M., An, M., Liu, L., Wang, H., ... & Wang, H. (2018). Tandem bispecific neutralizing antibody eliminates HIV-1 infection in humanized mice. The Journal of clinical investigation, 128(6), 2239-2251.

Cyt

Figure 2 Neutralizing activity of BiIA-DG and BiIA-SG.

Figure 2 Neutralizing activity of BiIA-DG and BiIA-SG.

The graph shows the IC90 values of BiIA-SG compared with parental IA-Hu5A8 against monoresistant viruses. Each pseudovirus was tested in duplicate.

Wu, X., Guo, J., Niu, M., An, M., Liu, L., Wang, H., ... & Wang, H. (2018). Tandem bispecific neutralizing antibody eliminates HIV-1 infection in humanized mice. The Journal of clinical investigation, 128(6), 2239-2251.

WB

Figure 3 Design and characterization of 5 IAs.

Figure 3 Design and characterization of 5 IAs.

The released IAs in culture supernatants were detected by Western blot analysis under reducing conditions.

Wu, X., Guo, J., Niu, M., An, M., Liu, L., Wang, H., ... & Wang, H. (2018). Tandem bispecific neutralizing antibody eliminates HIV-1 infection in humanized mice. The Journal of clinical investigation, 128(6), 2239-2251.

Neut

Figure 4 Design and characterization of 5 IAs.

Figure 4 Design and characterization of 5 IAs.

Each IA displayed neutralizing activity against pseudovirus HIV-1ADA.

Wu, X., Guo, J., Niu, M., An, M., Liu, L., Wang, H., ... & Wang, H. (2018). Tandem bispecific neutralizing antibody eliminates HIV-1 infection in humanized mice. The Journal of clinical investigation, 128(6), 2239-2251.


Specifications

  • Immunogen
  • Human CD4 molecule
  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Human IgG4
  • Specificity
  • CD4
  • Species Reactivity
  • Human
  • Clone
  • Hu5A8
  • Applications
  • ELISA, Cyt, WB, Neut

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Cytotoxicity, Western Blot and Neutralization.

Target

  • Alternative Names
  • CD4; CD4 molecule; CD4mut; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4Leu-3;

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Human Antibody

CAT Product Name Application Type
TAB-039 Anti-Human CD4 Recombinant Antibody (Zanolimumab) Neut, ELISA, IF, IP, FuncS, FC, IHC IgG1 - kappa

Chimeric Antibody

CAT Product Name Application Type
TAB-171 Anti-Human CD4 Recombinant Antibody (Keliximab) FuncS, IF, Neut, ELISA, FC, IP, ICC IgG1 - lambda
TAB-732 Anti-CD4 Recombinant Antibody (Clenoliximab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG4 - lambda
TAB-167LC-S(P) Anti-Human CD4 Recombinant Antibody scFv Fragment (cM-T412) ELISA Chimeric antibody (mouse/human)
TAB-170LC-S(P) Rat Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-170LC-S(P)) ELISA, SPR Rat scFv
TAB-170LC-F(E) Human Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-170LC-F(E)) ELISA, SPR Chimeric antibody (rat/human)

Immunotoxin

CAT Product Name Application Type
AGTO-G078E Anti-CD4 immunotoxin M-T151 (IgG)-PE Cytotoxicity assay, Function study
AGTO-G078R Anti-CD4 immunotoxin M-T151 (IgG)-RTA Cytotoxicity assay, Function study

Humanized Antibody

Fab Glycosylation

CAT Product Name Application Type
Gly-025LC Recombinant Anti-Human CD4 Antibody (Fab glycosylation) ELISA Humanized antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-351CQ Mouse Anti-CD4 Recombinant Antibody (clone 10C12) ELISA, WB, IF, Block Mouse IgG1
NEUT-352CQ Mouse Anti-CD4 Recombinant Antibody (clone 13B8.2) Block, ELISA, FC, IHC-Fr, WB Mouse IgG1, κ
NEUT-355CQ Mouse Anti-CD4 Recombinant Antibody (clone RPA-T4) FC, CyTOF, Block, IHC Mouse IgG1, κ
NEUT-357CQ Rat Anti-CD4 Recombinant Antibody (clone MAB0810) Block, Depletion, Stim Rat IgG2b
NEUT-358CQ Rat Anti-CD4 Recombinant Antibody (clone RM4-5) FC, CyTOF, Depletion, Block, IHC Rat IgG2a, κ

Neutralizing Antibody

CAT Product Name Application Type
NEUT-353CQ Mouse Anti-CD4 Recombinant Antibody (clone CBL976) FC, Neut, IF, IHC Mouse IgG1, κ
NEUT-354CQ Recombinant Mouse Anti-CD4 Antibody (CT-4) Neut, FC, IHC, IHC-Fr, IHC-P, IP IgG1, κ
NEUT-356CQ Mouse Anti-CD4 Recombinant Antibody (clone 3-4F4) Neut, FC, IHC, IHC-Fr, IP, ICC Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0560 Hi-Affi™ Rabbit Anti-CD4 Recombinant Antibody (clone DS560AB) IHC-P Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-039 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Zanolimumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-164 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Priliximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-260 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Tregalizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-171 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Keliximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-107 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Cedelizumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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