Human Anti-CD4 Recombinant Antibody (clone Hu5A8); scFv Fragment
CAT#: PSBL-424
Recombinant Humanized Antibody (Hu5A8) scFv Fragment is specific to CD4. This scFv fragment derived from a mouse antibody, 5A8, which binds D2 of CD4 and blocks HIV-1 infection in vitro.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (HEL, HL-60, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4
❮
❯
Specifications
- Immunogen
- Human CD4 molecule
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human scFv
- Specificity
- CD4
- Species Reactivity
- Human
- Clone
- Hu5A8
- Applications
- Block, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Outstanding Dependability
The fact that the lots are consistent is good. Every batch of my experiments has yielded reliable and repeatable results.
Robust Conjugation Principles
This antibody underwent a flawless conjugation process when combined with multiple fluorescent dyes. The FACS data were clear and simple to interpret.
Simple Situation Handling
Working with this antibody has been a breeze. Thawing and aliquoting on ice have gone perfectly thanks to the guidelines provided.
Q&As
-
Is it possible to utilize an antibody in multiplex tests?
A: It is dependent upon how well the multiplex assay's detection technology and antibodies work together.
-
How can I make sure my antibody is specific?
A: Make use of controls like peptide blocking studies, isotype controls, and no-primary controls.
-
Is there a species for which an antibody is not specified on the datasheet?
A: Though there's no assurance, it might work. Using the antibody for the species for which it has been validated is ideal.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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