The antibody was generated using spleen cells from mice that were immunized with P815 mastocytoma cells transfected with human CD4 cDNA. The mice were further boosted with recombinant CD4. The antibody exhibited a strong affinity for CD4 receptor-bearing CEM cells and displayed a binding specificity similar to that of a murine anti-CD4 monoclonal antibody called MT151.
Figure 1 PBMC (1 x 10⁵ cells/well) were cultured with PWM at a final concentration of 1 μg/ml at 37°C in the presence of serially-diluted CD4 mAbs, fragments, or isotype-matched controls.
After 7 days, culture supernatant was harvested and assayed for human IgM by solid phase ELISA. Percent inhibition of IgM production was based on the concentration of IgM in the supernatant of PWM-stimulated cells incubated without CD4 mAb. Isotypematched control antibodies did not inhibit IgM production.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
Figure 2 PBMC (3x 10⁵ cells/well) were cultured with PHA (2 μg/ml). CD4 mAbs, mAb fragments, or isotype-matched control antibodies were added at a final concentration of 10 μg/ml.
Plates were incubated for 3 days and culture supernatant was assayed for slL-2R by ELISA. Percent inhibition of slL-2R production was calculated by comparison with production in the absence of any mAb. Isotype-matched control antibodies, murine lgG2a (R11D10), chimeric lgG1 or lgG4 (c7E3), produced no inhibition. The average level of slL-2R produced by stimulated cells was 80 ng/10⁶ cells.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
Figure 3 PBMC (2x 10⁵ cells/well) were incubated for 3 days with 10 U/ml of rhlL-2 in microtiter plates coated with the murine CD3 mAb, M-T301 (5 μg/ml).
CD4 mAbs, fragments, or isotype-matched control mAbs were added at a final concentration of 10 μg/ml. Cells were pulsed for 18 h with 2 μCi/well of [³H]thymidine and harvested. Percent inhibition of [³H]thymidine uptake in the presence of antibody was based on c.p.m. incorporated in the absence of CD4 mAb. Isotype-matched control antibodies did not inhibit PMBC proliferation.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
Figure 4 PBMC (1 x 10⁵ cells/well) were cultured with 2 x 10⁵ cells/well irradiated (3500 rad) allogeneic lymphocytes.
CD4 mAbs, fragments, or isotypematched control mAbs were added at a final concentration of 10 μg/ml and plates were incubated for 6 days. Cells were pulsed for 18 h with 2 μCi/well [³H]thymidine and harvested. Incorporated counts were measured in a liquid scintillation spectrometer. Percent inhibition of [³H]thymidine uptake in the presence of antibody was based on c.p.m. incorporated in the absence of CD4 mAb. Isotype-matched control antibodies did not inhibit PBMC proliferation.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
Figure 5 PBMC (2 x 10⁵ cells/well) were cultured with tetanus toxoid at a final concentration of 5 μg/ml.
Serial 4-fold dilutions of CD4 mAbs, fragments, or isotype-matched controls were added and plates were incubated for 6 days. Cells were pulsed for 18 h with 2 μCi/well [³H]thymidine and harvested. Incorporated counts were measured in a liquid scintillation spectrometer. Percent inhibition of [³H]thymidine uptake in the presence of antibody was based on c.p.m. incorporated in the absence of CD4 mAb. Isotype-matched control antibodies did not inhibit PBMC proliferation.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
Figure 6 PBMC (2x 10⁵ cells/well) were incubated at 37°C with 10 μg/ml of CD4 mAb for 0, 5, or 24 h.
Cells were stained with FITC-labeled rabbit anti-mouse or anti-human IgG to detect the CD4 antigen and co-expression of the CD3 antigen was measured by staining with biotinyiated anti-Leu4, followed by streptavidin-PE. At each time point, the percent of cells staining positive for FITC or PE, or for both fluorophores, was determined by flow cytometry on a FACScan (Becton-Dickinson). The murine CD4 mAb, M-T413, served as a positive control.
Dalesandro, M. R., Pak, K. Y., Tam, S., Wilson, E., Looney, J. E., Knight, D. M.,... & Daddona, P. E. (1993). Effects of isotype and Fc region on in vitro function of a mouse/human chimeric CD4 antibody. International immunology, 5(3), 283-291.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-039 | Anti-Human CD4 Recombinant Antibody (Zanolimumab) | Neut, ELISA, IF, IP, FuncS, FC, IHC | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-164 | Anti-Human CD4 Recombinant Antibody (Priliximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
TAB-171 | Anti-Human CD4 Recombinant Antibody (Keliximab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG1 - lambda |
TAB-732 | Anti-CD4 Recombinant Antibody (Clenoliximab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG4 - lambda |
TAB-162LC-S(P) | Anti-Human CD4 Recombinant Antibody scFv Fragment | ELISA | Chimeric antibody (mouse/human) |
TAB-164LC-S(P) | Monkey Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-164LC-S(P)) | FC, SPR, ELISA | Monkey scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G078E | Anti-CD4 immunotoxin M-T151 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G078R | Anti-CD4 immunotoxin M-T151 (IgG)-RTA | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-033 | Human Anti-CD4 Recombinant Antibody (clone 17b); scFv Fragment | WB, ELISA, FuncS | Human scFv |
PSBL-034 | Mouse Anti-CD4 Recombinant Antibody (clone 2D5); scFv Fragment | Neut, ELISA, FuncS | Mouse scFv |
PSBL-035 | Mouse Anti-CD4 Recombinant Antibody (clone Q425); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
HPAB-2059LY-S(P) | Mouse Anti-CD4 Recombinant Antibody; scFv Fragment (HPAB-2059LY-S(P)) | FC | Mouse scfv |
HPAB-2112-FY-S(P) | Mouse Anti-CD4 Recombinant Antibody (clone MT15.1); scFv Fragment | Inhib | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-033 | Human Anti-CD4 Recombinant Antibody (clone 17b); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-034 | Mouse Anti-CD4 Recombinant Antibody (clone 2D5); Fab Fragment | Neut, ELISA, FuncS | Mouse Fab |
PFBL-035 | Mouse Anti-CD4 Recombinant Antibody (clone Q425); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
FAMAB-0054-YC-F(E) | Mouse Anti-CD4 Recombinant Antibody (clone M-T310); Fab Fragment | Neut | Mouse Fab |
FAMAB-0055-YC-F(E) | Mouse Anti-CD4 Recombinant Antibody (clone M-T404); Fab Fragment | Neut | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-161LC | Anti-Human CD4 Recombinant Antibody (gOKT4A-4) | ELISA | Humanized antibody |
TAB-168LC | Anti-Human CD4 Recombinant Antibody (TRX1) | ELISA | Humanized antibody |
TAB-171LC | Human Anti-CD4 Recombinant Antibody (TAB-171LC) | ELISA, FC, SPR | Humanized antibody |
TAB-172LC | Human Anti-CD4 Recombinant Antibody (TAB-172LC) | ELISA, Inhib | Human IgG |
TAB-168LC-S(P) | Anti-Human CD4 Recombinant Antibody scFv Fragment (TRX1) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-160LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (OKT4A) | ELISA | |
TAB-166LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (M-T412) | ELISA | |
TAB-169LC-F(E) | Rat Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-169LC-F(E)) | ELISA, SPR | Rat Fab |
TAB-173LC-F(E) | Anti-Human CD4 Recombinant Antibody Fab Fragment (IgA F425-Alg8) | ELISA | |
TAB-174LC-F(E) | Mouse Anti-CD4 Recombinant Antibody; Fab Fragment (TAB-174LC-F(E)) | ELISA, FC, IH | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-025LC | Recombinant Anti-Human CD4 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-352CQ | Mouse Anti-CD4 Recombinant Antibody (clone 13B8.2) | Block, ELISA, FC, IHC-Fr, WB | Mouse IgG1, κ |
NEUT-355CQ | Mouse Anti-CD4 Recombinant Antibody (clone RPA-T4) | FC, CyTOF, Block, IHC | Mouse IgG1, κ |
NEUT-357CQ | Rat Anti-CD4 Recombinant Antibody (clone MAB0810) | Block, Depletion, Stim | Rat IgG2b |
NEUT-358CQ | Rat Anti-CD4 Recombinant Antibody (clone RM4-5) | FC, CyTOF, Depletion, Block, IHC | Rat IgG2a, κ |
NEUT-359CQ | Rat Anti-CD4 Recombinant Antibody (clone YTS177) | Block | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-353CQ | Mouse Anti-CD4 Recombinant Antibody (clone CBL976) | FC, Neut, IF, IHC | Mouse IgG1, κ |
NEUT-354CQ | Recombinant Mouse Anti-CD4 Antibody (CT-4) | Neut, FC, IHC, IHC-Fr, IHC-P, IP | IgG1, κ |
NEUT-356CQ | Mouse Anti-CD4 Recombinant Antibody (clone 3-4F4) | Neut, FC, IHC, IHC-Fr, IP, ICC | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0560 | Hi-Affi™ Rabbit Anti-CD4 Recombinant Antibody (clone DS560AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0758-CN | Human Anti-CD4 Recombinant Antibody (clone Variant B) | ELISA, FC | Human IgG1 |
HPAB-0759-CN | Human Anti-CD4 Recombinant Antibody (clone Variant D) | ELISA, FC | Human IgG1 |
HPAB-0792-CN | Mouse Anti-CD4 Recombinant Antibody (clone B4) | ELISA | Mouse IgG |
HPAB-0793-CN | Human Anti-CD4 Recombinant Antibody (clone chB4) | ELISA | Chimeric (mouse/human) IgG1 |
HPAB-0794-CN | Human Anti-CD4 Recombinant Antibody (clone dB4C7) | ELISA | Chimeric (mouse/human) IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-039 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Zanolimumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-164 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Priliximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-260 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Tregalizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-171 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Keliximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-107 | Afuco™ Anti-CD4 ADCC Recombinant Antibody (Cedelizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
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