What does the isotype of an antibody mean?

The antibody's effector activities, such as complement fixation and binding to Fc receptors, are influenced by its isotype.

How can I make my assay more sensitive?

Boost the assay parameters, employ a more sensitive detection technique, or increase the antibody concentration.

What does the presence of biotinylation in an antibody mean?

The streptavidin-biotin interactions that enable detection or enrichment are made possible by the covalent attachment of biotin to a biotinylated antibody.

Human Anti-CD4 Recombinant Antibody (clone Hu5A8); Fab Fragment

CAT#: PFBL-424

Recombinant Humanized Antibody (Hu5A8) Fab Fragment is specific to CD4. This Fab fragment derived from a mouse antibody, 5A8, which binds D2 of CD4 and blocks HIV-1 infection in vitro.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11/if_selected.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HEL, HL-60, NB-4, THP-1, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4

❮

❯

Specifications

Immunogen
Human CD4 molecule

Host Species
Human

Derivation
Humanized

Type
Human Fab

Specificity
CD4

Species Reactivity
Human

Clone
Hu5A8

Applications
Block, Neut

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD4; CD4 molecule; CD4mut; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4Leu-3

Gene ID
920

UniProt ID
B4DT49

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Fast results

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Practical packaging

The practical and secure packaging of this antibody product makes it easy to handle and store, minimizing the risk of damage or contamination.

Respectable brand

This product comes from a reputable brand known for their high-quality antibodies. We have not been disappointed.

Q&As

What does the isotype of an antibody mean?

A: The antibody's effector activities, such as complement fixation and binding to Fc receptors, are influenced by its isotype.
How can I make my assay more sensitive?

A: Boost the assay parameters, employ a more sensitive detection technique, or increase the antibody concentration.
What does the presence of biotinylation in an antibody mean?

A: The streptavidin-biotin interactions that enable detection or enrichment are made possible by the covalent attachment of biotin to a biotinylated antibody.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PFBL-424, RRID: AB_3111716)

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PFBL-424.pdf

MSDS

MSDS - PFBL-424.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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