Anti-Human CD4 Recombinant Antibody (OKT4A) (CAT#: TAB-160LC)

Figure 1 Increase in p56lck tyrosine kinase activity in THP-1 cells by anti-CD4 antibody and gp120.

THP-1 cells were incubated with OKT4A antibody at 2.0 mg/ml or gp120 at 2.0 mg/ml for 4 h. The cells were lysed in buffer containing sodium orthovanadate and NaF, and the protein concentration was measured. Equal amounts of proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-phosphotyrosine monoclonal antibody. MW, molecular weight.

Hui, R., Curtis, J. F., Sumner, M. T., Shears, S. B., Glasgow, W. C., & Eling, T. E. (1995). Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages. Journal of virology, 69(12), 8020-8026.

Figure 2 Western analysis for PGHS expression in THP-1 monocytes

The cells were harvested, and the proteins were isolated, separated, and transferred to nitrocellulose. The blots were immunoreacted with murine anti-PGHS-1 (A) or anti-human PGHS-2 (B) and developed by ECL techniques. "Standard" refers to human recombinant PGHS-1 or PGHS-2. MW, molecular weight.

Hui, R., Curtis, J. F., Sumner, M. T., Shears, S. B., Glasgow, W. C., & Eling, T. E. (1995). Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages. Journal of virology, 69(12), 8020-8026.

Figure 3 Binding of CD4 mutants to a variety of anti-CD4 mAbs.

Only the CD4 region ofthe gels is shown. Autoradiographic exposure times for different mutants were adjusted to compensate for differences in expression levels. WT, wild type.

Mizukami, T., Fuerst, T. R., Berger, E. A., & Moss, B. (1988). Binding region for human immunodeficiency virus (HIV) and epitopes for HIV-blocking monoclonal antibodies of the CD4 molecule defined by site-directed mutagenesis. Proceedings of the National Academy of Sciences, 85(23), 9273-9277.