Anti-CD4 Recombinant Antibody (Clenoliximab) (CAT#: TAB-732)

Recombinant chimeric (primate/human) antibody expressed in CHO binding to human CD4/p55. Clenoliximab is a monoclonal antibody against CD4. It acts as an immunomodulator and has been investigated for the treatment of rheumatoid arthritis. The drug is a chimeric antibody from Macaca irus and Homo sapiens.


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  • Published Data
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Inhib

Figure 1 Fc receptor binding characteristics of keliximab and clenoliximab.

Figure 1 Fc receptor binding characteristics of keliximab and clenoliximab.

A, Keliximab-mediated adhesion of IFN-treated monocytic cell line (THP1) to CD4 fibroblast transfectants. Also shown is the inhibition of this CD4 mAb-mediated adhesion by sCD4 along with the effect of truncated form of keliximab. B, Comparison of IgG1 and IgG4 anti-CD4 mAbs in FcR-CD4-mediated cell-cell adhesion assay. Percent adhesion of THP-1 cells to CD4 T cells was calculated as: (fluorescence of test samples/total fluorescence) 100. Data points are the mean SEM of triplicate cultures.

Reddy, M. P., Kinney, C. A. S., Chaikin, M. A., Payne, A., Fishman-Lobell, J., Tsui, P.,... & Reff, M. (2000). Elimination of Fc receptor-dependent effector functions of a modified IgG4 monoclonal antibody to human CD4. The Journal of Immunology, 164(4), 1925-1933.

FC

Figure 2 Flow cytometric analysis.

Figure 2 Flow cytometric analysis.

A, Keliximab and clenoliximab-mediated adhesion of CD4 T cells to IFN-induced Fc receptor-expressing monocytes. Anti-CD4 mAbs serve as a bridge between the monocytes on the microtiter plates and fluorescent dye loaded SupT1-18 T cells. Percent binding of SupT1-18 cells to monocytes was calculated as in Fig. 4B (fluorescence of test samples/total fluorescence) 100. Data represent one of three experiments that showed identical results (15). B, Freshly isolated human monocytes were cultured for 36 h with 250 U/ml human IFN-, after which induction of Fc receptor expression was measured by staining with anti-human CD64-FITC, CD32-FITC, and CD16-FITC Abs. Flow cytometric analysis was performed using CellQuest software (Becton Dickinson).

Reddy, M. P., Kinney, C. A. S., Chaikin, M. A., Payne, A., Fishman-Lobell, J., Tsui, P.,... & Reff, M. (2000). Elimination of Fc receptor-dependent effector functions of a modified IgG4 monoclonal antibody to human CD4. The Journal of Immunology, 164(4), 1925-1933.

FC

Figure 3 Keliximab and clenoliximab induced CD4 and CD3 receptor modulation.

Figure 3 Keliximab and clenoliximab induced CD4 and CD3 receptor modulation.

Shown are flow cytometric two-dimensional scatter plots of human PBMCs incubated with 100 ng/ml of keliximab or clenoliximab or medium without added Ab and dual stained for CD4 (OKT4) and CD3 markers after 24 h of culture.

Reddy, M. P., Kinney, C. A. S., Chaikin, M. A., Payne, A., Fishman-Lobell, J., Tsui, P.,... & Reff, M. (2000). Elimination of Fc receptor-dependent effector functions of a modified IgG4 monoclonal antibody to human CD4. The Journal of Immunology, 164(4), 1925-1933.

ELISA

Figure 4 Cytokine secretion profile.Animals were administered 1*10¹¹ particles of Ad-lacZ intratracheally in the presence or the absence of Clenoliximab (CD4 mAb), and splenocytes were harvested 14 days later for stimulation with adenovirus and analysis for secretion of cytokines.

Figure 4 Cytokine secretion profile.Animals were administered 1*10¹¹ particles of Ad-lacZ intratracheally in the presence or the absence of Clenoliximab (CD4 mAb), and splenocytes were harvested 14 days later for stimulation with adenovirus and analysis for secretion of cytokines.

IL-2, IFN-g, IL-4, and IL-10 were measured in serum by ELISA and plotted as picograms per milliliter. The results were obtained from pooled spleens of three to five mice, and the values shown are the mean 6 1 SD from three or four separate experiments. Supernatants were analyzed from cells treated with medium (f) or stimulated with Ad-lacZ (M). These analyses were performed with muCD4KO, HuCD4, or HuCD4 treated with Clenoliximab (CD4 mAb). p, p, 0.001, using Student's t test.

Chirmule, N., Truneh, A., Haecker, S. E., Tazelaar, J., Gao, G. P., Raper, S. E.,... & Wilson, J. M. (1999). Repeated administration of adenoviral vectors in lungs of human CD4 transgenic mice treated with a nondepleting CD4 antibody. The Journal of Immunology, 163(1), 448-455.

Stim

Figure 5 Cellular and humoral immune functions in HuCD4 mice treated with Clenoliximab (CD4 mAb).

Figure 5 Cellular and humoral immune functions in HuCD4 mice treated with Clenoliximab (CD4 mAb).

This figure summarizes the T cell proliferative and humoral responses in HuCD4 mice treated with multiple doses of vector in the presence or the absence of Clenoliximab. A and B, Splenocytes obtained on various days were analyzed for adenovirus- and SEB-induced lymphoproliferative responses as described in Materials and Methods. Data are presented as the stimulation index, which is the incorporation of radioactivity in the presence of stimulator (i.e., adenovirus or SEB) over the incorporation of isotope when cells are incubated with medium alone. Analyses were performed on days 28, 60, and 90. A, Adenovirus-induced lymphoproliferation (LPR) responses; B, SEB-induced LPR responses. C and D, The humoral immune responses of animals that received multiple administrations of vector in the presence or the absence of Clenoliximab. C, Neutralizing Ab from serum; D, neutralizing Ab from BAL (both presented as reciprocal dilutions).

Chirmule, N., Truneh, A., Haecker, S. E., Tazelaar, J., Gao, G. P., Raper, S. E.,... & Wilson, J. M. (1999). Repeated administration of adenoviral vectors in lungs of human CD4 transgenic mice treated with a nondepleting CD4 antibody. The Journal of Immunology, 163(1), 448-455.

IHC

Figure 6 Treatment of HuCD4 mice with Clenoliximab prolongs transgene expression in mouse lung.

Figure 6 Treatment of HuCD4 mice with Clenoliximab prolongs transgene expression in mouse lung.

Animals were euthanized on days 4, 28, 60, and 90 (rows 1, 2, 3, and 4, respectively) and analyzed for expression of b-galactosidase by X-galactosidase histochemistry. Magni-
fication is 3200. The results are representative of one of six mice in each group.

Chirmule, N., Truneh, A., Haecker, S. E., Tazelaar, J., Gao, G. P., Raper, S. E.,... & Wilson, J. M. (1999). Repeated administration of adenoviral vectors in lungs of human CD4 transgenic mice treated with a nondepleting CD4 antibody. The Journal of Immunology, 163(1), 448-455.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • primate
  • Derivation
  • Chimeric (primate/human)
  • Type
  • IgG4 - lambda
  • Species Reactivity
  • Human
  • Applications
  • IF, IP, Neut, FuncS, ELISA, FC, ICC, Inhib, Stim, IHC
  • Trade name
  • primatized
  • CAS
  • 182912-58-9
  • Generic Name
  • clenoliximab
  • Related Disease
  • Asthma

Product Property

  • Purity
  • >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.

Applications

  • Application Notes
  • The CD4 antibody has been reported in applications of IF, IP, Neut, FuncS, ELISA, FC, ICC, Inhib, Stim, IHC.

Target

  • Alternative Names
  • clenoliximab;primatized;182912-58-9;IDEC-151;BB-217969;CD4;CD4 molecule;CD4 antigen (p55) , T cell surface glycoprotein CD4;T-cell surface glycoprotein CD4;CD4 receptor;CD4 antigen (p55);T-cell surface antigen T4/Leu-3;CD4mut;

Related Resources

  • Biosimilar Overview
  • Related Diseases
Please refer to Clenoliximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Clenoliximab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clenoliximab"

Afuco™ Anti-CD4 ADCC Recombinant Antibody (Clenoliximab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant chimeric (primate/human) antibody expressed in CHO binding to human CD4/p55. Clenoliximab is a monoclonal antibody against CD4. It acts as an immunomodulator and has been investigated for the treatment of rheumatoid arthritis. The drug is a chimeric antibody from Macaca irus and Homo sapiens.

See other products for "CD4"

Human Antibody

CAT Product Name Application Type
TAB-039 Anti-Human CD4 Recombinant Antibody (Zanolimumab) Neut, ELISA, IF, IP, FuncS, FC, IHC IgG1 - kappa

Immunotoxin

CAT Product Name Application Type
AGTO-G078E Anti-CD4 immunotoxin M-T151 (IgG)-PE Cytotoxicity assay, Function study
AGTO-G078R Anti-CD4 immunotoxin M-T151 (IgG)-RTA Cytotoxicity assay, Function study

Chimeric Antibody

CAT Product Name Application Type
TAB-167LC Anti-Human CD4 Recombinant Antibody (cM-T412) ELISA, Inhib Chimeric antibody (mouse/human)
TAB-170LC Human Anti-CD4 Recombinant Antibody (TAB-170LC) ELISA, FC, ADCC, CDC, SPR Chimeric antibody (rat/human)
TAB-165LC-S(P) Monkey Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-165LC-S(P)) FC, SPR, ELISA Monkey scFv
TAB-167LC-S(P) Anti-Human CD4 Recombinant Antibody scFv Fragment (cM-T412) ELISA Chimeric antibody (mouse/human)
TAB-170LC-S(P) Rat Anti-CD4 Recombinant Antibody; scFv Fragment (TAB-170LC-S(P)) ELISA, SPR Rat scFv

Fab Glycosylation

CAT Product Name Application Type
Gly-025LC Recombinant Anti-Human CD4 Antibody (Fab glycosylation) ELISA Humanized antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-351CQ Mouse Anti-CD4 Recombinant Antibody (clone 10C12) ELISA, WB, IF, Block Mouse IgG1
NEUT-352CQ Mouse Anti-CD4 Recombinant Antibody (clone 13B8.2) Block, ELISA, FC, IHC-Fr, WB Mouse IgG1, κ
NEUT-355CQ Mouse Anti-CD4 Recombinant Antibody (clone RPA-T4) FC, CyTOF, Block, IHC Mouse IgG1, κ
NEUT-357CQ Rat Anti-CD4 Recombinant Antibody (clone MAB0810) Block, Depletion, Stim Rat IgG2b
NEUT-358CQ Rat Anti-CD4 Recombinant Antibody (clone RM4-5) FC, CyTOF, Depletion, Block, IHC Rat IgG2a, κ

Neutralizing Antibody

CAT Product Name Application Type
NEUT-353CQ Mouse Anti-CD4 Recombinant Antibody (clone CBL976) FC, Neut, IF, IHC Mouse IgG1, κ
NEUT-354CQ Recombinant Mouse Anti-CD4 Antibody (CT-4) Neut, FC, IHC, IHC-Fr, IHC-P, IP IgG1, κ
NEUT-356CQ Mouse Anti-CD4 Recombinant Antibody (clone 3-4F4) Neut, FC, IHC, IHC-Fr, IP, ICC Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0560 Hi-Affi™ Rabbit Anti-CD4 Recombinant Antibody (clone DS560AB) IHC-P Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-039 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Zanolimumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-164 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Priliximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-260 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Tregalizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-171 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Keliximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-107 Afuco™ Anti-CD4 ADCC Recombinant Antibody (Cedelizumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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