Anti-Human CD4 Recombinant Antibody (15A7) (CAT#: TAB-175LC)

Figure 1 The inhibition of HIV-1 infection by mAb 15A7.

The HIV-1 isolate HIVNL4-3 (clade B) was analyzed for inhibition using the anti-CD4 monoclonal antibody 15A7 and its fragments using a luciferase-based virus neutralization assay in TZM-bl cells. The data are represented as the percent inhibition relative to the concentration of 15A7 and its fragments. The experiment was repeated four times to generate mean value and standard deviation.

Hou, W., Fang, C., Liu, J., Yu, H., Qi, J., Zhang, Z.,... & Li, S. (2015). Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody. Antiviral research, 122, 101-111.

Figure 2 mAb 15A7 binds to the D1 domain of CD4.

15A7-labeled cell lines detected via immunofluorescence, using a control anti-p24 mAb. 15A7 binds with the CD4 positive cell lines in the immunofluorescence images. The DAPI-stained nuclei are shown in blue, and 15A7 recognizing CD4 is visualized using a FITC-labeled goat anti-mouse antibody in green.

Hou, W., Fang, C., Liu, J., Yu, H., Qi, J., Zhang, Z.,... & Li, S. (2015). Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody. Antiviral research, 122, 101-111.

Figure 3 The binding sites of mAb 15A7 mapped via mutagenesis.

The dot-ELISA assay for the serially truncated CD4 D1 domains that were displayed on HBV-C149 particles. Decreased concentrations of 15A7 were applied to determine the concentration-dependent reaction. HBcAg, CD4-D1 and CD4-D1D2 were used as controls.

Hou, W., Fang, C., Liu, J., Yu, H., Qi, J., Zhang, Z.,... & Li, S. (2015). Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody. Antiviral research, 122, 101-111.

Figure 4 The binding sites of mAb 15A7 mapped via mutagenesis.

Site-directed mutations of the gp120 binding sites were conducted on the CD4-D1D2 protein. The resultant proteins, F43A, K46A, R58A, R59A and N73A, were resolved in non-reducing and reducing SDS-PAGE and reacted with 15A7 via western blot. F43A, K46A, R59A and N73A lost binding capacity with 15A7, demonstrating that 15A7 recognizes a region that overlaps with the gp120 binding site on CD4.

Hou, W., Fang, C., Liu, J., Yu, H., Qi, J., Zhang, Z.,... & Li, S. (2015). Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody. Antiviral research, 122, 101-111.