Recombinant Mouse Anti-E. coli LPS Antibody (WN1 222-5) (CAT#: PABL-078)
Recombinant Mouse Antibody (WN1 222-5) is capable of binding to E. coli LPS, expressed in Chinese Hamster Ovary cells (CHO).
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Figure 1 Interaction of WN1 222-5 with LPS and core oligosaccharides conjugated to BSA.
ELISA binding curves of the interaction of WN1 222-5 at different concentrations with LPS (A) and core oligosaccharides (for structures, see Fig. 1) conjugated to BSA (B) of E. coli F470 (R1), F576 (R2), F653 (R3), 2513 (R4), and S. enterica sv. Minnesota (R1) at a concentration of immobilized ligand of 50 pmol (●), 25 pmol (▲), 12.5 pmol (■), 6.25 pmol (◆), 3.125 pmol (○), 1.56 pmol (△), 0.79 pmol (□), and 0.39 pmol (◇).
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
Figure 2 Interaction of WN1 222-5 with core oligosaccharides of E. coli J-5 conjugated to BSA.
ELISA binding curves of the interaction of WN1 222-5 at different concentrations with neoglycoconjugates of E. coli J-5 core oligosaccharides (for structures, see Fig. 2). A–E, heptasaccharide P4, octasaccharide P4, heptasaccharide P3, octasaccharide 1 P3, and nonasaccharide P3, respectively, coated at ligand concentrations of 50 pmol (●), 25 pmol (▲), 12.5 pmol (■), 6.25 pmol (◆), 3.125 pmol (○), 1.56 pmol (△), 0.79 pmol (□), and 0.39 pmol (◇).
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
Figure 3 ITC microcalorimetry of WN1 222-5 binding to the E. coli F2513 (R4 core) oligosaccharide at 150 mM (A) and 300 mM (B) salt concentration.
N represents the number of binding sites; KA and KD are affinity and dissociation constants, respectively; and H is the enthalpy of binding.
Müller-Loennies, S., Brade, L., MacKenzie, C. R., Di Padova, F. E., & Brade, H. (2003). Identification of a cross-reactive epitope widely present in lipopolysaccharide from enterobacteria and recognized by the cross-protective monoclonal antibody WN1 222-5. Journal of Biological Chemistry, 278(28), 25618-25627.
Specifications
- Immunogen
- Escherichia coli Lipopolysaccarides
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native E. coli LPS
- Species Reactivity
- E. coli
- Clone
- WN1 222-5
- Applications
- WB, ELISA, FuncS, ITC
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA and ITC. For details, refer to published data.
Target
- Alternative Names
- Lipopolysaccarides; LPS
Related Resources
Rheumatoid Arthritis
Graft-versus-Host Disease
TLR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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(Creative Biolabs Cat# PABL-078, RRID: AB_3111625)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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