This is a chimeric mouse-human monoclonal antibody (cMAb) against Burkholderia pseudomallei (BP). The cMAb CK3 reacts with lipopolysaccharides (38∼52 kDa in B. pseudomallei; 38∼60 kDa in B. thailandensis). This chimeric antibody was produced from hybridoma clone BP1 7F7.
Figure 1 Binding property of 3 cMAbs produced by CHO-DG44 cells without DHFR amplification.
For ELISA, the pIRES-H(L)-DHFR vectors containing chimeric H-chain (H1, H2, and H3) or L-chain (L1, L2, and L3) were cotransfected into CHO-DG44 cells using a LF-2000 reagent and the master seed cells were incubated for 3 days to get sufficient amounts of cMAbs before the DHFR-amplification process.
Figure 2 Western blot analysis of BP, BM and BT with 3 cMAbs.
For Western blot analysis, 3 cMAb culture supernatants (cMAbs CK1, CK2, and CK3) were used at 1:5 dilutions.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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