Anti-Human GM2 Recombinant Antibody (BIW-8962) (CAT#: TAB-320CL)

Figure 1 Expression levels of ganglioside GM2 were evaluated in malignant pleural mesothelioma cell lines.

Cells were detached and incubated with BIW-8962 antibody on ice for 30 min. Bound Abs were detected with FITC conjugated goat anti-human IgG Abs.

Li, Q., Wang, W., Machino, Y., Yamada, T., Kita, K., Oshima, M., ... & Iida, S. (2015). Therapeutic activity of glycoengineered anti‐GM 2 antibodies against malignant pleural mesothelioma. Cancer science, 106(1), 102-107.

Figure 2 Anti-GM2 antibody BIW-8962 showed therapeutic activity in an MSTO-211H orthotropic mouse model.

MSTO-211H malignant pleural mesothelioma cells were inoculated into the thoracic cavity in SCID mice, and the animals were treated with BIW-8962 and ⁄ or human peripheral blood mononuclear cells (MNC). The mice were then i.v. administered BIW-8962 and ⁄ or MNC on days 7 and 14. At 3 weeks after tumor cell inoculation, the mice were sacrificed and their tumor weights were measured. The bars represent the mean of the group data. *P < 0.05 and **P < 0.01 between each treatment and the control groups.

Li, Q., Wang, W., Machino, Y., Yamada, T., Kita, K., Oshima, M., ... & Iida, S. (2015). Therapeutic activity of glycoengineered anti‐GM 2 antibodies against malignant pleural mesothelioma. Cancer science, 106(1), 102-107.

Figure 3 Ganglioside GM2 expression in clinical malignant pleural mesothelioma specimens.

Frozen sections obtained from 26 mesothelioma patients were incubated in DAPI at room temperature for 20 min. After washing, the sections were incubated in 5% FBS–PBS for 10 min for blocking and subsequently incubated in 30 lg ⁄ mL BIW-8962 or anti-dinitrophenol antibodies conjugated with Alexa Fluor 488 at 4 °C for 6 h.

Li, Q., Wang, W., Machino, Y., Yamada, T., Kita, K., Oshima, M., ... & Iida, S. (2015). Therapeutic activity of glycoengineered anti‐GM 2 antibodies against malignant pleural mesothelioma. Cancer science, 106(1), 102-107.