Anti-HCMV gB Recombinant Antibody (7H3) (CAT#: MRO-1040LC)

Figure 1 Plaque formation and leukocyte transfer inhibition by human monoclonal and serum antibodies.

Dose-effect inhibition of HCMV plaque formation (PFI) in ARPE-19 cells, and HCMV transfer to leukocytes (LTI) from infected HUVEC cells by serial concentrations of human mAbs specific for different sites on the pentamer complex. An anti-gB mAb (7H3) was used as a control.

Fetal Human Cytomegalovirus Transmission Correlates with Delayed Maternal Antibodies to gH/gL/pUL128-130-131 Complex during Primary Infection

Figure 2 Immunofluorescent staining of VR1814-infected ARPE-19 epithelial cells at 96 h p.i. HCMV fluorescent nuclei (p72) are shown in green, while Evans blue counterstaining is shown in red.

MAbs to the pentamer complex and its pUL128L components completely blocked syncytium formation (SF) at the higher concentration tested (10 µg/ml), unlike mAbs to gO, gH, or gB, which only partially inhibited SF.

The pentameric complex of human Cytomegalovirus: cell tropism, virus dissemination, immune response and vaccine development

Figure 3 shows cross-competition experiments in which HEK293T cells transfected with hCMV gB gene were first incubated with an unlabeled competitor antibody followed by staining with a biotinylated anti-gH or anti-gB antibody.