Anti-Human CD4 Recombinant Antibody (Ibalizumab) (CAT#: TAB-146)

Figure 1 The epitope of ibalizumab is likely to be conformational.

(A) Binding of ibalizumab to soluble hCD4, CD4-IgG2, PR14 (aa 121 to 134), or TG26 (aa 115 to 140) as measured by ELISA. (B) Competition for ibalizumab binding to sCD4 in solution by CD4-IgG2 or PR14 as measured by ELISA. (C) Binding titers of mouse anti-PR14 antiserum to hCD4 or PR14. (D) FACS analysis of cell surface staining by ibalizumab in control 293T cells or cells transfected with hD2-mCD4 or hCD4 plasmid DNA.

Song, R., Franco, D., Kao, C. Y., Yu, F., Huang, Y., & Ho, D. D. (2010). Epitope mapping of ibalizumab, a humanized anti-CD4 monoclonal antibody with anti-HIV-1 activity in infected patients. Journal of virology, 84(14), 6935-6942.

Figure 2 Comparison of ibalizumab with M-T441 in virus neutralization and competitive binding to hCD4.

(A) Neutralizing activity of ibalizumab versus M-T441 as tested against four HIV-1 isolates (JRCSF, WT-1a, MDR-1a, and MDR-5a). (B) Two-way binding competitions between ibalizumab and M-T441 based on FACS analysis.

Song, R., Franco, D., Kao, C. Y., Yu, F., Huang, Y., & Ho, D. D. (2010). Epitope mapping of ibalizumab, a humanized anti-CD4 monoclonal antibody with anti-HIV-1 activity in infected patients. Journal of virology, 84(14), 6935-6942.

Figure 1 Anti-Human CD4 Therapeutic Antibody (Ibalizumab) (TAB-146-L) in WB

Western blot analysis of TAB-146 was performed by loading Recombinant Human CD4 Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-146-L and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 2 Anti-Human CD4 Therapeutic Antibody (Ibalizumab) (TAB-146) in ELISA

ELISA analysis of TAB-146 was performed by coating with Recombinant Human CD4 Protein. Then blocked with BSA, and incubated with Anti-Human CD4 Antibody (TAB-146). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.